Swap and stop – kinetochores play error correction with microtubules

Harinath Doodhi (Lead / Corresponding author), Tomoyuki Tanaka (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

Correct chromosome segregation in mitosis relies on chromosome bi-orientation, in which sister kinetochores attach to microtubules from opposite spindle poles prior to segregation. To establish bi-orientation, aberrant kinetochore–microtubule interactions must be resolved through the error correction process. During error correction, kinetochore–microtubule interactions are exchanged (swapped) if aberrant, but the exchange must stop when biorientation is established. In this article, we discuss recent findings in budding yeast, which have revealed fundamental molecular mechanisms promoting this ‘swap and stop’ process for error correction. Where relevant, we also compare the findings in budding yeast with mechanisms in higher eukaryotes. Evidence suggests that Aurora B kinase differentially regulates kinetochore attachments to the microtubule end and its lateral side and switches relative strength of the two kinetochore–microtubule attachment modes, which drives the exchange of kinetochore–microtubule interactions to resolve aberrant interactions. However, Aurora B kinase, recruited to centromeres and inner kinetochores, cannot reach its targets at kinetochore-microtubule interface when tension causes kinetochore stretching, which stops the kinetochore–microtubule exchange once bi-orientation is established.
Original languageEnglish
Article number2100246
Number of pages12
JournalBioEssays
Volume44
Issue number5
Early online date8 Mar 2022
DOIs
Publication statusPublished - May 2022

Keywords

  • Dam1 complex
  • INCENP
  • Ndc80 complex
  • aurora B kinase
  • chromosome biorientation
  • chromosome segregation
  • error correction
  • kinetochore–microtubule interactions

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