Swap and stop – kinetochores play error correction with microtubules

Harinath Doodhi (Lead / Corresponding author), Tomoyuki U. Tanaka (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)
    59 Downloads (Pure)

    Abstract

    Correct chromosome segregation in mitosis relies on chromosome bi-orientation, in which sister kinetochores attach to microtubules from opposite spindle poles prior to segregation. To establish bi-orientation, aberrant kinetochore–microtubule interactions must be resolved through the error correction process. During error correction, kinetochore–microtubule interactions are exchanged (swapped) if aberrant, but the exchange must stop when biorientation is established. In this article, we discuss recent findings in budding yeast, which have revealed fundamental molecular mechanisms promoting this ‘swap and stop’ process for error correction. Where relevant, we also compare the findings in budding yeast with mechanisms in higher eukaryotes. Evidence suggests that Aurora B kinase differentially regulates kinetochore attachments to the microtubule end and its lateral side and switches relative strength of the two kinetochore–microtubule attachment modes, which drives the exchange of kinetochore–microtubule interactions to resolve aberrant interactions. However, Aurora B kinase, recruited to centromeres and inner kinetochores, cannot reach its targets at kinetochore-microtubule interface when tension causes kinetochore stretching, which stops the kinetochore–microtubule exchange once bi-orientation is established.
    Original languageEnglish
    Article number2100246
    Number of pages12
    JournalBioEssays
    Volume44
    Issue number5
    Early online date8 Mar 2022
    DOIs
    Publication statusPublished - May 2022

    Keywords

    • Dam1 complex
    • INCENP
    • Ndc80 complex
    • aurora B kinase
    • chromosome biorientation
    • chromosome segregation
    • error correction
    • kinetochore–microtubule interactions

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology

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