Projects per year
Abstract
Correct chromosome segregation in mitosis relies on chromosome bi-orientation, in which sister kinetochores attach to microtubules from opposite spindle poles prior to segregation. To establish bi-orientation, aberrant kinetochore–microtubule interactions must be resolved through the error correction process. During error correction, kinetochore–microtubule interactions are exchanged (swapped) if aberrant, but the exchange must stop when biorientation is established. In this article, we discuss recent findings in budding yeast, which have revealed fundamental molecular mechanisms promoting this ‘swap and stop’ process for error correction. Where relevant, we also compare the findings in budding yeast with mechanisms in higher eukaryotes. Evidence suggests that Aurora B kinase differentially regulates kinetochore attachments to the microtubule end and its lateral side and switches relative strength of the two kinetochore–microtubule attachment modes, which drives the exchange of kinetochore–microtubule interactions to resolve aberrant interactions. However, Aurora B kinase, recruited to centromeres and inner kinetochores, cannot reach its targets at kinetochore-microtubule interface when tension causes kinetochore stretching, which stops the kinetochore–microtubule exchange once bi-orientation is established.
Original language | English |
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Article number | 2100246 |
Number of pages | 12 |
Journal | BioEssays |
Volume | 44 |
Issue number | 5 |
Early online date | 8 Mar 2022 |
DOIs | |
Publication status | Published - May 2022 |
Keywords
- Dam1 complex
- INCENP
- Ndc80 complex
- aurora B kinase
- chromosome biorientation
- chromosome segregation
- error correction
- kinetochore–microtubule interactions
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
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Kinetochore-Microtubule Interactions: Steps Towards Bi-Orientation (Investigator Award)
Tanaka, T. (Investigator)
1/12/20 → 31/12/26
Project: Research
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SELFCC: Chromosome Self-clearing Completes Sister Chromatid Separation
Tanaka, T. (Investigator)
COMMISSION OF THE EUROPEAN COMMUNITIES
1/04/13 → 30/09/18
Project: Research
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Molecular Mechanisms Regulating the Kinetochore-Microtubule Interaction in Mitosis (Principal Research Fellowship)
Tanaka, T. (Investigator)
1/04/12 → 30/04/21
Project: Research