Synchronised cycling gene oscillations in presomitic mesoderm cells require cell-cell contact

Miguel Maroto, J. Kim Dale, Mary-Lee Dequeant, Anne-Cecile Petit, Olivier Pourquie

    Research output: Contribution to journalArticle

    63 Citations (Scopus)

    Abstract

    Segmentation of the vertebrate body axis is initiated early in development with the sequential formation of somites. Somitogenesis is temporally regulated by a molecular oscillator, the segmentation clock, which acts within presomitic mesoderm (PSM) cells to drive periodic expression of the cyclic genes. We have investigated the kinetics of the progression of cycling gene expression along the PSM. Here we show that c-hairy1 and c-hairy2 mRNA expression traverses the PSM in an entirely progressive manner and that both these genes and c-Lfng maintain a similar anterior limit of expression during each cycle. However, some differences are seen regarding both the onset of a new oscillation of these genes and the duration of their expression in the caudal PSM. We also investigated whether oscillating cyclic gene expression in the PSM is entirely cell autonomous. We find that while small PSM explants are still able to maintain their oscillation schedule, once they are dissociated, PSM cells are no longer able to maintain synchronous oscillations. The results imply that cell communication or a community effect is essential for the normal pattern of cyclic gene expression in these cells.
    Original languageEnglish
    Pages (from-to)309-315
    Number of pages7
    JournalInternational Journal of Developmental Biology
    Volume49
    Issue number2-3
    DOIs
    Publication statusPublished - 2005

    Keywords

    • Cyclic gene
    • Gene expression
    • Oscillation

    Fingerprint Dive into the research topics of 'Synchronised cycling gene oscillations in presomitic mesoderm cells require cell-cell contact'. Together they form a unique fingerprint.

  • Cite this