Synergy between two transcription factors directs gene expression in Dictyostelium tip-organiser cells

Hong-Yu Wang, Jeffrey G. Williams

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    cotC requires the transcription factor CudA for its expression in the posterior, prespore cells of the slug, while the expL7 gene requires CudA for its expression in the anterior, tip-organiser region. In order to identify additional transcription factors that might mediate tip-organiser specific expression, we performed affinity chromatography on slug nuclear extracts. The affinity matrix bore cap-site distal sequences from region A' of the expL7 promoter; an essential region located upstream of the CudA binding domain. One of the proteins purified was G-box binding factor (GBF), a zinc finger transcription factor which binds to G-rich elements, known as G boxes, that are present in the promoters of many developmental genes, including cotC. Previous work identified an essential sequence motif within region A' and we show that this element is a G box, that binds recombinant GBF. Moreover, a G box from within the cotC promoter can substitute for region A' of expL7 in directing tip-organiser specific expression of expL7. Thus the same two transcription factors, CudA and GBF, seem to co-operate to direct both tip-organiser and prespore gene expression. How then is specificity achieved? Replacing a CudA binding region in the cotCpromoter with the CudA binding domain from expL7strongly represses cotCpromoter activity. Hence we suggest that differences in the topology of the multiple CudA half- sites contained within the two different CudA binding regions, coupled with differences in the signalling environment between tip-organiser cells and prespore cells, ensure correct expL7 expression.

    Original languageEnglish
    Pages (from-to)1301-1307
    Number of pages7
    JournalInternational Journal of Developmental Biology
    Volume54
    Issue number8-9
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Dictyostelium
    • cudA
    • GBF
    • Tip-organiser
    • Prespore
    • Transcription factor
    • Camp-inducible gene
    • Discoideum
    • Element
    • Binding
    • Identification
    • Sufficient
    • Receptor
    • Family
    • Switch
    • Stata

    Cite this

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    title = "Synergy between two transcription factors directs gene expression in Dictyostelium tip-organiser cells",
    abstract = "cotC requires the transcription factor CudA for its expression in the posterior, prespore cells of the slug, while the expL7 gene requires CudA for its expression in the anterior, tip-organiser region. In order to identify additional transcription factors that might mediate tip-organiser specific expression, we performed affinity chromatography on slug nuclear extracts. The affinity matrix bore cap-site distal sequences from region A' of the expL7 promoter; an essential region located upstream of the CudA binding domain. One of the proteins purified was G-box binding factor (GBF), a zinc finger transcription factor which binds to G-rich elements, known as G boxes, that are present in the promoters of many developmental genes, including cotC. Previous work identified an essential sequence motif within region A' and we show that this element is a G box, that binds recombinant GBF. Moreover, a G box from within the cotC promoter can substitute for region A' of expL7 in directing tip-organiser specific expression of expL7. Thus the same two transcription factors, CudA and GBF, seem to co-operate to direct both tip-organiser and prespore gene expression. How then is specificity achieved? Replacing a CudA binding region in the cotCpromoter with the CudA binding domain from expL7strongly represses cotCpromoter activity. Hence we suggest that differences in the topology of the multiple CudA half- sites contained within the two different CudA binding regions, coupled with differences in the signalling environment between tip-organiser cells and prespore cells, ensure correct expL7 expression.",
    keywords = "Dictyostelium, cudA, GBF, Tip-organiser, Prespore, Transcription factor, Camp-inducible gene, Discoideum, Element, Binding, Identification, Sufficient, Receptor, Family, Switch, Stata",
    author = "Hong-Yu Wang and Williams, {Jeffrey G.}",
    year = "2010",
    doi = "10.1387/ijdb.103141hw",
    language = "English",
    volume = "54",
    pages = "1301--1307",
    journal = "International Journal of Developmental Biology",
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    }

    Synergy between two transcription factors directs gene expression in Dictyostelium tip-organiser cells. / Wang, Hong-Yu; Williams, Jeffrey G.

    In: International Journal of Developmental Biology, Vol. 54, No. 8-9, 2010, p. 1301-1307.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Synergy between two transcription factors directs gene expression in Dictyostelium tip-organiser cells

    AU - Wang, Hong-Yu

    AU - Williams, Jeffrey G.

    PY - 2010

    Y1 - 2010

    N2 - cotC requires the transcription factor CudA for its expression in the posterior, prespore cells of the slug, while the expL7 gene requires CudA for its expression in the anterior, tip-organiser region. In order to identify additional transcription factors that might mediate tip-organiser specific expression, we performed affinity chromatography on slug nuclear extracts. The affinity matrix bore cap-site distal sequences from region A' of the expL7 promoter; an essential region located upstream of the CudA binding domain. One of the proteins purified was G-box binding factor (GBF), a zinc finger transcription factor which binds to G-rich elements, known as G boxes, that are present in the promoters of many developmental genes, including cotC. Previous work identified an essential sequence motif within region A' and we show that this element is a G box, that binds recombinant GBF. Moreover, a G box from within the cotC promoter can substitute for region A' of expL7 in directing tip-organiser specific expression of expL7. Thus the same two transcription factors, CudA and GBF, seem to co-operate to direct both tip-organiser and prespore gene expression. How then is specificity achieved? Replacing a CudA binding region in the cotCpromoter with the CudA binding domain from expL7strongly represses cotCpromoter activity. Hence we suggest that differences in the topology of the multiple CudA half- sites contained within the two different CudA binding regions, coupled with differences in the signalling environment between tip-organiser cells and prespore cells, ensure correct expL7 expression.

    AB - cotC requires the transcription factor CudA for its expression in the posterior, prespore cells of the slug, while the expL7 gene requires CudA for its expression in the anterior, tip-organiser region. In order to identify additional transcription factors that might mediate tip-organiser specific expression, we performed affinity chromatography on slug nuclear extracts. The affinity matrix bore cap-site distal sequences from region A' of the expL7 promoter; an essential region located upstream of the CudA binding domain. One of the proteins purified was G-box binding factor (GBF), a zinc finger transcription factor which binds to G-rich elements, known as G boxes, that are present in the promoters of many developmental genes, including cotC. Previous work identified an essential sequence motif within region A' and we show that this element is a G box, that binds recombinant GBF. Moreover, a G box from within the cotC promoter can substitute for region A' of expL7 in directing tip-organiser specific expression of expL7. Thus the same two transcription factors, CudA and GBF, seem to co-operate to direct both tip-organiser and prespore gene expression. How then is specificity achieved? Replacing a CudA binding region in the cotCpromoter with the CudA binding domain from expL7strongly represses cotCpromoter activity. Hence we suggest that differences in the topology of the multiple CudA half- sites contained within the two different CudA binding regions, coupled with differences in the signalling environment between tip-organiser cells and prespore cells, ensure correct expL7 expression.

    KW - Dictyostelium

    KW - cudA

    KW - GBF

    KW - Tip-organiser

    KW - Prespore

    KW - Transcription factor

    KW - Camp-inducible gene

    KW - Discoideum

    KW - Element

    KW - Binding

    KW - Identification

    KW - Sufficient

    KW - Receptor

    KW - Family

    KW - Switch

    KW - Stata

    U2 - 10.1387/ijdb.103141hw

    DO - 10.1387/ijdb.103141hw

    M3 - Article

    VL - 54

    SP - 1301

    EP - 1307

    JO - International Journal of Developmental Biology

    JF - International Journal of Developmental Biology

    SN - 0214-6282

    IS - 8-9

    ER -