Synergy of peptide and sugar in O-GlcNAcase substrate recognition

Marianne Schimpl, Vladimir S. Borodkin, Lindsey J. Gray, Daan M. F. van Aalten

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Protein O-GlcNAcylation is an essential reversible posttranslational modification in higher eukaryotes. O-GlcNAc addition and removal is catalyzed by O-GlcNAc transferase and O-GlcNAcase, respectively. We report the molecular details of the interaction of a bacterial O-GlcNAcase homolog with three different synthetic glycopeptides derived from characterized O-GlcNAc sites in the human proteome. Strikingly, the peptides bind a conserved O-GlcNAcase substrate binding groove with similar orientation and conformation. In addition to extensive contacts with the sugar, O-GlcNAcase recognizes the peptide backbone through hydrophobic interactions and intramolecular hydrogen bonds, while avoiding interactions with the glycopeptide side chains. These findings elucidate the molecular basis of O-GlcNAcase substrate specificity, explaining how a single enzyme achieves cycling of the complete O-GlcNAc proteome. In addition, this work will aid development of O-GlcNAcase inhibitors that target the peptide binding site.
Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalChemistry & Biology
Volume19
Issue number2
DOIs
Publication statusPublished - 24 Feb 2012

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