Synthesis and characterization of an iodinated bile-salt derivative for photoaffinity-labeling

Colin J. Henderson, Ian W. Percy-Robb

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The synthesis and characterisation of a novel iodinated bile salt derivative, 125I-labelled 3ß-azidocholylhistamine, is described. The derivative is handled by rat liver in a similar manner to taurocholate and binding to bovine serum albumin, a well-characterised bile acid-binding protein, is demonstrated. The suitability of the derivative for photoaffinity labelling is assessed.
    Original languageEnglish
    Pages (from-to)257-264
    Number of pages8
    JournalBiochimica et Biophysica Acta. Lipids and Lipid Metabolism
    Volume795
    Issue number2
    DOIs
    Publication statusPublished - 12 Sep 1984

    Keywords

    • Bile salt
    • Photoaffinity labelling
    • Rat liver

    Cite this

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    title = "Synthesis and characterization of an iodinated bile-salt derivative for photoaffinity-labeling",
    abstract = "The synthesis and characterisation of a novel iodinated bile salt derivative, 125I-labelled 3{\ss}-azidocholylhistamine, is described. The derivative is handled by rat liver in a similar manner to taurocholate and binding to bovine serum albumin, a well-characterised bile acid-binding protein, is demonstrated. The suitability of the derivative for photoaffinity labelling is assessed.",
    keywords = "Bile salt, Photoaffinity labelling, Rat liver",
    author = "Henderson, {Colin J.} and Percy-Robb, {Ian W.}",
    year = "1984",
    month = "9",
    day = "12",
    doi = "10.1016/0005-2760(84)90073-0",
    language = "English",
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    journal = "Biochimica et Biophysica Acta. Lipids and Lipid Metabolism",
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    publisher = "Elsevier BV",
    number = "2",

    }

    Synthesis and characterization of an iodinated bile-salt derivative for photoaffinity-labeling. / Henderson, Colin J.; Percy-Robb, Ian W.

    In: Biochimica et Biophysica Acta. Lipids and Lipid Metabolism, Vol. 795, No. 2, 12.09.1984, p. 257-264.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Synthesis and characterization of an iodinated bile-salt derivative for photoaffinity-labeling

    AU - Henderson, Colin J.

    AU - Percy-Robb, Ian W.

    PY - 1984/9/12

    Y1 - 1984/9/12

    N2 - The synthesis and characterisation of a novel iodinated bile salt derivative, 125I-labelled 3ß-azidocholylhistamine, is described. The derivative is handled by rat liver in a similar manner to taurocholate and binding to bovine serum albumin, a well-characterised bile acid-binding protein, is demonstrated. The suitability of the derivative for photoaffinity labelling is assessed.

    AB - The synthesis and characterisation of a novel iodinated bile salt derivative, 125I-labelled 3ß-azidocholylhistamine, is described. The derivative is handled by rat liver in a similar manner to taurocholate and binding to bovine serum albumin, a well-characterised bile acid-binding protein, is demonstrated. The suitability of the derivative for photoaffinity labelling is assessed.

    KW - Bile salt

    KW - Photoaffinity labelling

    KW - Rat liver

    U2 - 10.1016/0005-2760(84)90073-0

    DO - 10.1016/0005-2760(84)90073-0

    M3 - Article

    VL - 795

    SP - 257

    EP - 264

    JO - Biochimica et Biophysica Acta. Lipids and Lipid Metabolism

    JF - Biochimica et Biophysica Acta. Lipids and Lipid Metabolism

    SN - 0005-2760

    IS - 2

    ER -