Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose

Greg B.G. Moorhead, Timothy A.J. Haystead, Carol MacKintosh

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

Original languageEnglish
Title of host publicationProtein Phosphatase Protocols
EditorsGreg Moorhead
PublisherSpringer
Pages39-45
Number of pages7
ISBN (Print)9781588297112
DOIs
Publication statusPublished - 1 Dec 2007

Publication series

NameMethods in Molecular Biology
Volume365
ISSN (Print)1064-3745

Keywords

  • affinity chromatography
  • biotin
  • cyanobacteria
  • cyclic-peptide
  • hepatotoxin
  • Microcystin
  • okadaic acid
  • protein phosphatase

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