Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
|Title of host publication||Protein Phosphatase Protocols|
|Number of pages||7|
|Publication status||Published - 1 Dec 2007|
|Name||Methods in Molecular Biology|
- affinity chromatography
- okadaic acid
- protein phosphatase