Synthesis and use of the protein phosphatase affinity matrices microcystin-sepharose and microcystin-biotin-sepharose

Greg B.G. Moorhead, Timothy A.J. Haystead, Carol MacKintosh

    Research output: Chapter in Book/Report/Conference proceedingChapter

    9 Citations (Scopus)

    Abstract

    Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.

    Original languageEnglish
    Title of host publicationProtein Phosphatase Protocols
    EditorsGreg Moorhead
    PublisherSpringer
    Pages39-45
    Number of pages7
    ISBN (Print)9781588297112
    DOIs
    Publication statusPublished - 1 Dec 2007

    Publication series

    NameMethods in Molecular Biology
    Volume365
    ISSN (Print)1064-3745

    Keywords

    • affinity chromatography
    • biotin
    • cyanobacteria
    • cyclic-peptide
    • hepatotoxin
    • Microcystin
    • okadaic acid
    • protein phosphatase

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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