Synthesis of 1′-(4′-Thio-β-d-Ribofuranosyl) Uracil

4'-Thionucleosides, where the furanose ring oxygen is replaced with sulfur, have attracted significant interest due to their biological activity and metabolic stability. In recent years, a growing number of reports have detailed the chemical synthesis and evaluation of 4'-thio nucleoside analogs, alongside methodologies for the provision of thiosugar-containing scaffolds. The original report for synthesizing was made by Imbach and coworkers in their synthesis of 4'-thio-ß-d-oligoribonucleotides and utilized as the starting material, recording a 38% yield for the two-step process. NMR analysis confirmed the correct structure notably with the presence of signals for the alkene in the nucleobase with vicinal coupling to each other.