Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: a potent tool for the inhibition/isolation of serine esterases and proteases

Adrian P. Higson, Michael A. J. Ferguson, Andrei V. Nikolaev

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of a-chymotrypsin.

    Original languageEnglish
    Pages (from-to)407-409
    Number of pages3
    JournalSynthesis-Stuttgart
    Issue number3
    DOIs
    Publication statusPublished - Mar 1999

    Keywords

    • Biotinylated analogue of diisopropylfluorophosphate
    • 6-N-biotinylaminohexanol
    • H-phosphonate condensation
    • Halide exchange
    • Biotin

    Cite this