Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: a potent tool for the inhibition/isolation of serine esterases and proteases

Adrian P. Higson; Michael A. J. Ferguson; Andrei V. Nikolaev

doi:10.1055/s-1999-3419

Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: a potent tool for the inhibition/isolation of serine esterases and proteases

Adrian P. Higson
, Michael A. J. Ferguson
, Andrei V. Nikolaev

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of a-chymotrypsin.

Original language	English
Pages (from-to)	407-409
Number of pages	3
Journal	Synthesis-Stuttgart
Issue number	3
DOIs	https://doi.org/10.1055/s-1999-3419
Publication status	Published - Mar 1999

Keywords

Biotinylated analogue of diisopropylfluorophosphate
6-N-biotinylaminohexanol
H-phosphonate condensation
Halide exchange
Biotin

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10.1055/s-1999-3419

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title = "Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: a potent tool for the inhibition/isolation of serine esterases and proteases",

abstract = "6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of a-chymotrypsin.",

keywords = "Biotinylated analogue of diisopropylfluorophosphate, 6-N-biotinylaminohexanol, H-phosphonate condensation, Halide exchange, Biotin",

author = "Higson, \{Adrian P.\} and Ferguson, \{Michael A. J.\} and Nikolaev, \{Andrei V.\}",

year = "1999",

month = mar,

doi = "10.1055/s-1999-3419",

language = "English",

pages = "407--409",

journal = "Synthesis-Stuttgart",

issn = "0039-7881",

publisher = "Georg Thieme Verlag",

number = "3",

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TY - JOUR

T1 - Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate

T2 - a potent tool for the inhibition/isolation of serine esterases and proteases

AU - Higson, Adrian P.

AU - Ferguson, Michael A. J.

AU - Nikolaev, Andrei V.

PY - 1999/3

Y1 - 1999/3

N2 - 6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of a-chymotrypsin.

AB - 6-N-Biotinylaminohexyl isopropyl phosphorofluoridate 2 was prepared by treatment of the corresponding hydrogen phosphonic diester with triethylamine trihydrofluoride and iodine. The activity of the phosphorofluoridate was tested by the inhibition of a-chymotrypsin.

KW - Biotinylated analogue of diisopropylfluorophosphate

KW - 6-N-biotinylaminohexanol

KW - H-phosphonate condensation

KW - Halide exchange

KW - Biotin

U2 - 10.1055/s-1999-3419

DO - 10.1055/s-1999-3419

M3 - Article

SN - 0039-7881

SP - 407

EP - 409

JO - Synthesis-Stuttgart

JF - Synthesis-Stuttgart

IS - 3

ER -

Synthesis of 6-N-biotinylaminohexyl isopropyl phosphorofluoridate: a potent tool for the inhibition/isolation of serine esterases and proteases

Abstract

Keywords

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