Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme

Shinya Harusawa; Kensuke Fujii; Masayoshi Nishiura; Lisa Araki; Yoshihide Usami; Zheng-yun Zhao; David M. J. Lilley

doi:10.3987/COM-11-12263

Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme

Shinya Harusawa, Kensuke Fujii, Masayoshi Nishiura, Lisa Araki, Yoshihide Usami, Zheng-yun Zhao, David M. J. Lilley

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Synthesis of C4-linked imidazole N-pivaloyloxymethyl (P0M)-2'-O-cyanoethylated (CE) C-1- and C-3-ribonucleoside phosphoramidites 1a and 1b is described. These phosphoramidite products were incorporated into RNA sequence through solid phase phosphoramidite approach, providing RNA with imidazole linked through different length to sugar residue, to study the mechanism of a ribozyme.

Original language	English
Pages (from-to)	2041-2055
Number of pages	15
Journal	Heterocycles
Volume	83
Issue number	9
DOIs	https://doi.org/10.3987/COM-11-12263
Publication status	Published - 1 Sept 2011

Keywords

Imidazole
Phosphoramidite
Ribozyme
RNA
Mechanism
RIBONUCLEOSIDE PHOSPHORAMIDITE
ACTIVE-SITE
RNA

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10.3987/COM-11-12263

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title = "Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme",

abstract = "Synthesis of C4-linked imidazole N-pivaloyloxymethyl (P0M)-2'-O-cyanoethylated (CE) C-1- and C-3-ribonucleoside phosphoramidites 1a and 1b is described. These phosphoramidite products were incorporated into RNA sequence through solid phase phosphoramidite approach, providing RNA with imidazole linked through different length to sugar residue, to study the mechanism of a ribozyme.",

keywords = "Imidazole, Phosphoramidite, Ribozyme, RNA, Mechanism, RIBONUCLEOSIDE PHOSPHORAMIDITE, ACTIVE-SITE, RNA",

author = "Shinya Harusawa and Kensuke Fujii and Masayoshi Nishiura and Lisa Araki and Yoshihide Usami and Zheng-yun Zhao and Lilley, {David M. J.}",

year = "2011",

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doi = "10.3987/COM-11-12263",

language = "English",

volume = "83",

pages = "2041--2055",

journal = "Heterocycles",

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TY - JOUR

T1 - Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme

AU - Harusawa, Shinya

AU - Fujii, Kensuke

AU - Nishiura, Masayoshi

AU - Araki, Lisa

AU - Usami, Yoshihide

AU - Zhao, Zheng-yun

AU - Lilley, David M. J.

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Synthesis of C4-linked imidazole N-pivaloyloxymethyl (P0M)-2'-O-cyanoethylated (CE) C-1- and C-3-ribonucleoside phosphoramidites 1a and 1b is described. These phosphoramidite products were incorporated into RNA sequence through solid phase phosphoramidite approach, providing RNA with imidazole linked through different length to sugar residue, to study the mechanism of a ribozyme.

AB - Synthesis of C4-linked imidazole N-pivaloyloxymethyl (P0M)-2'-O-cyanoethylated (CE) C-1- and C-3-ribonucleoside phosphoramidites 1a and 1b is described. These phosphoramidite products were incorporated into RNA sequence through solid phase phosphoramidite approach, providing RNA with imidazole linked through different length to sugar residue, to study the mechanism of a ribozyme.

KW - Imidazole

KW - Phosphoramidite

KW - Ribozyme

KW - RNA

KW - Mechanism

KW - RIBONUCLEOSIDE PHOSPHORAMIDITE

KW - ACTIVE-SITE

KW - RNA

U2 - 10.3987/COM-11-12263

DO - 10.3987/COM-11-12263

M3 - Article

SN - 0385-5414

VL - 83

SP - 2041

EP - 2055

JO - Heterocycles

JF - Heterocycles

IS - 9

ER -

Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme

Abstract

Keywords

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