TY - JOUR
T1 - Synthesis of N-benzyloxycarbonyl-l-cysteinylglycine 3-dimethylaminopropylamide disulfide
T2 - A cheap and convenient new assay for trypanothione reductase
AU - El-Waer, Abdussalam
AU - Douglas, Kenneth T.
AU - Smith, Keith
AU - Fairlamb, Alan H.
PY - 1991/10
Y1 - 1991/10
N2 - Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi.
AB - Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi.
UR - http://www.scopus.com/inward/record.url?scp=0026000158&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(91)90531-W
DO - 10.1016/0003-2697(91)90531-W
M3 - Article
C2 - 1789428
AN - SCOPUS:0026000158
SN - 0003-2697
VL - 198
SP - 212
EP - 216
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -