Synthesis of N-benzyloxycarbonyl-l-cysteinylglycine 3-dimethylaminopropylamide disulfide: A cheap and convenient new assay for trypanothione reductase

TY - JOUR

T1 - Synthesis of N-benzyloxycarbonyl-l-cysteinylglycine 3-dimethylaminopropylamide disulfide

T2 - A cheap and convenient new assay for trypanothione reductase

AU - El-Waer, Abdussalam

AU - Douglas, Kenneth T.

AU - Smith, Keith

AU - Fairlamb, Alan H.

PY - 1991/10

Y1 - 1991/10

N2 - Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi.

AB - Trypanothione disulfide [N1,N8-bis(glutathionyl)-spermidine], the physiological substrate for the chemotherapeutic target enzyme trypanothione reductase, is difficult to isolate, expensive to buy, and awkward to synthesize. Here we describe the straightforward synthesis of N,N′-bis(benzyloxycarbonyl)-l-cysteinylglycyl-3-dimethylaminopropylamide disulfide, which is shown to be a good alternative substrate for the trypanothione reductases from Crithidia fasciculata and Trypanosoma cruzi.

UR - https://www.scopus.com/pages/publications/0026000158

U2 - 10.1016/0003-2697(91)90531-W

DO - 10.1016/0003-2697(91)90531-W

M3 - Article

C2 - 1789428

AN - SCOPUS:0026000158

SN - 0003-2697

VL - 198

SP - 212

EP - 216

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -