Synthetic biology of B cell activation: understanding signal amplification at the B cell antigen receptor using a rebuilding approach

Yogesh Kulathu, Christa Zuern, Jianying Yang, Michael Reth (Lead / Corresponding author)

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology approach, we dissect the roles of different ITAM residues of Igα in Syk activation. We find that a leucine to glycine mutation at the Y+3 position after the first ITAM tyrosine prevents Syk binding and activation. However, a preactivated Syk can still phosphorylate this tyrosine in trans. Our data show that the formation of a Syk/ITAM initiation complex and trans-ITAM phosphorylation is crucial for BCR signal amplification. In contrast, the interaction of Lyn with the first ITAM tyrosine is not altered by the leucine to glycine mutation. In addition, our study suggests that an ITAM-bound Syk phosphorylates the non-ITAM tyrosine Y204 of Igα only in cis. Collectively, our reconstitution experiments suggest a model whereby first trans-phosphorylation amplifies the BCR signal and subsequently cis-phosphorylation couples the receptor to downstream signaling elements.

Original languageEnglish
JournalBiological Chemistry
Early online date22 Nov 2018
DOIs
Publication statusPublished - Nov 2018

Keywords

  • BCR
  • ITAM
  • Lyn
  • signal amplification
  • Syk
  • synthetic biology

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