Systemic modulation of gene expression in tomato by Trichoderma hamatum 382

G. Alfano, M. L. Lewis Ivey, C. Cakir, J. I. B. Bos, S. A. Miller, L. V. Madden, S. Kamoun, H. A. J. Hoitink

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    Abstract

    A light sphagnum peat mix inoculated with Trichoderma hamatum 382 consistently provided a significant (P = 0.05) degree of protection against bacterial spot of tomato and its pathogen Xanthomonas euvesicatoria 110c compared with the control peat mix, even though this biocontrol agent did not colonize aboveground plant parts. To gain insight into the mechanism by which T hamatum 382 induced resistance in tomato, high-density oligonucleotide microarrays were used to determine its effect on the expression pattern of 15,925 genes in leaves just before they were inoculated with the pathogen. T hamatum 382 consistently modulated the expression of genes in tomato leaves. We identified 45 genes to be differentially expressed across the replicated treatments, and 4 1 of these genes could be assigned to at least one of seven functional categories. T homatum 382-induced genes have functions associated with biotic or abiotic stress, as well as RNA, DNA, and protein metabolism. Four extensin and extensin-like proteins were induced. However, besides pathogenesis-related protein 5, the main markers of systemic acquired resistance were not significantly induced. This work showed that T hamatum 382 actively induces systemic changes in plant physiology and disease resistance through systemic modulation of the expression of stress and metabolism genes.

    Original languageEnglish
    Pages (from-to)429-437
    Number of pages9
    JournalPhytopathology
    Volume97
    Issue number4
    DOIs
    Publication statusPublished - Apr 2007

    Keywords

    • INDUCTION
    • BIOCONTROL AGENT
    • ISR
    • X. campestris pv. vesicatoria
    • COMPOST
    • microarray analysis
    • extensins
    • mechanism of induced resistance
    • Solanum lycopersicum
    • DEFENSE RESPONSES
    • Lycopersicon esculentum
    • CUCUMIS-SATIVUS
    • RESISTANCE
    • TRICHODERMA-HARZIANUM
    • ARABIDOPSIS
    • GROWTH-PROMOTING RHIZOBACTERIA
    • AMENDED POTTING MIXES

    Cite this

    Alfano, G., Lewis Ivey, M. L., Cakir, C., Bos, J. I. B., Miller, S. A., Madden, L. V., Kamoun, S., & Hoitink, H. A. J. (2007). Systemic modulation of gene expression in tomato by Trichoderma hamatum 382. Phytopathology, 97(4), 429-437. https://doi.org/10.1094/PHYTO-97-4-0429