TAF1B Is a TFIIB-Like Component of the Basal Transcription Machinery for RNA Polymerase I

Srivatsava Naidu, J. Karsten Friedrich, Jackie Russell, Joost C. B. M. Zomerdijk (Lead / Corresponding author)

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    40 Citations (Scopus)

    Abstract

    Transcription by eukaryotic RNA polymerases (Pols) II and III and archaeal Pol requires structurally related general transcription factors TFIIB, Brf1, and TFB, respectively, which are essential for polymerase recruitment and initiation events. A TFIIB-like protein was not evident in the Pol I basal transcription machinery. We report that TAF1B, a subunit of human Pol I basal transcription factor SL1, is structurally related to TFIIB/TFIIB-like proteins, through predicted amino-terminal zinc ribbon and cyclin-like fold domains. SL1, essential for Pol I recruitment to the ribosomal RNA gene promoter, also has an essential postpolymerase recruitment role, operating through TAF1B. Therefore, a TFIIB-related protein is implicated in preinitiation complex assembly and postpolymerase recruitment events in Pol I transcription, underscoring the parallels between eukaryotic Pol I, II, and III and archaeal transcription machineries.

    Original languageEnglish
    Pages (from-to)1640-1642
    Number of pages3
    JournalScience
    Volume333
    Issue number6049
    DOIs
    Publication statusPublished - 16 Sep 2011

    Keywords

    • PREINITIATION COMPLEX
    • RDNA TRANSCRIPTION
    • ZINC RIBBON
    • FACTOR SL1
    • INITIATION
    • PROMOTER
    • SUBUNITS
    • RECRUITMENT
    • BINDING
    • TBP

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