Targeting signals for a bacterial Sec-independent export system direct plant thylakoid import by the Delta pH pathway

Margaret Wexler, Erik G Bogsch, Ralf Bernd Klosgen, Tracy Palmer, Colin Robinson, Ben C Berks

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    Preproteins targeted to the Sec-independent protein transport systems of plant thylakoids and of bacteria both have unusual transfer peptides bearing a consensus twin-arginine motif. Possible mechanistic similarity between the two Sec-independent transport pathways was investigated by assessing the ability of bacterial twin-arginine transfer peptides to direct thylakoid import. High efficiency import mas observed. This process was demonstrated to occur specifically via the Sec-independent Delta pH pathway and to depend on an intact twin-arginine motif on the transfer peptide. These results provide strong evidence for the operation of mechanistically related Sec-independent protein transport pathways in chloroplasts and bacteria. (C) 1998 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)339-342
    Number of pages4
    JournalFEBS Letters
    Issue number3
    Publication statusPublished - 24 Jul 1998

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