TY - JOUR
T1 - Technical validation studies of a dual-wavelength LED-based photoacoustic and ultrasound imaging system
AU - Joseph, James
AU - Ajith Singh, Mithun Kuniyil
AU - Sato, Naoto
AU - Bohndiek, Sarah E.
N1 - JJ and SEB received research funding from PreXion Corporation , which (Photoacoustic imaging division) was later acquired by CYBERDYNE INC. MKAS and NS are employees of CYBERDYNE INC. MKAS and NS contributed to the study design and assisted with image reconstruction. Final study design, data collection, analysis and interpretation were performed by JJ and SEB. Additional funding was provided through Cancer Research UK under grant numbers C14303/A17197 , C9545/A29580 , C47594/A16267 , and C197/A16465 as well as the EPSRC (EP/R003599/1). These additional sponsors had no involvement in the study design nor the collection, analysis and interpretation of data.
PY - 2021/6
Y1 - 2021/6
N2 - Recent advances in high power, pulsed, light emitting diodes (LEDs) have shown potential as fast, robust and relatively inexpensive excitation sources for photoacoustic imaging (PAI), yet systematic characterization of performance for biomedical imaging is still lacking. We report here technical and biological validation studies of a commercial dual-wavelength LED-based PAI and ultrasound system. Phantoms and small animals were used to assess temporal precision. In phantom studies, we found high temporal stability of the LED-based PAI system, with no significant drift in performance observed during 6 h of operation or over 30 days of repeated measurements. In vivo dual-wavelength imaging was able to map the dynamics of changes in blood oxygenation during oxygen-enhanced imaging and reveal the kinetics of indocyanine green contrast agent inflow after intravenous administration (Tmax∼6 min). Taken together, these studies indicate that LED-based excitation could be promising for future application in functional and molecular PAI.
AB - Recent advances in high power, pulsed, light emitting diodes (LEDs) have shown potential as fast, robust and relatively inexpensive excitation sources for photoacoustic imaging (PAI), yet systematic characterization of performance for biomedical imaging is still lacking. We report here technical and biological validation studies of a commercial dual-wavelength LED-based PAI and ultrasound system. Phantoms and small animals were used to assess temporal precision. In phantom studies, we found high temporal stability of the LED-based PAI system, with no significant drift in performance observed during 6 h of operation or over 30 days of repeated measurements. In vivo dual-wavelength imaging was able to map the dynamics of changes in blood oxygenation during oxygen-enhanced imaging and reveal the kinetics of indocyanine green contrast agent inflow after intravenous administration (Tmax∼6 min). Taken together, these studies indicate that LED-based excitation could be promising for future application in functional and molecular PAI.
KW - Light emitting diodes
KW - Phantoms
KW - Photoacoustics
UR - https://www.scopus.com/pages/publications/85104356428
U2 - 10.1016/j.pacs.2021.100267
DO - 10.1016/j.pacs.2021.100267
M3 - Article
C2 - 33948434
AN - SCOPUS:85104356428
SN - 2213-5979
VL - 22
JO - Photoacoustics
JF - Photoacoustics
M1 - 100267
ER -