Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

James Cobley; Nikos V. Margaritelis; Panagiotis N. Chatzinikolaou; Michalis G. Nikolaidis; Gareth W. Davison

doi:10.20944/preprints202405.0729.v1

Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

James Cobley (Lead / Corresponding author)
, Nikos V. Margaritelis
, Panagiotis N. Chatzinikolaou
, Michalis G. Nikolaidis
, Gareth W. Davison

Molecular Cell and Developmental Biology

Research output: Working paper/Preprint › Preprint

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Abstract

Formidable and often seemingly insurmountable conceptual, technical, and methodological challenges hamper the measurement of oxidative stress in humans. For instance, fraught and flawed methods, such as the thiobarbituric acid reactive substances assay kits for lipid peroxidation, rate-limit progress. To advance translational redox research, we present ten comprehensive “cheat codes” for measuring oxidative stress in humans. The cheat codes include analytical approaches to assess reactive oxygen species, antioxidants, biomarkers of oxidative damage and redox regulation. They provide essential conceptual, technical, and methodological information inclusive of curated “do” and “don’t” guidelines. Given the biochemical complexity of oxidative stress, we present a research question-grounded decision tree guide for selecting the most appropriate cheat code (s) to implement in a prospective human experiment. Worked examples demonstrate the benefits of the decision tree-based cheat code selection tool. The ten cheat codes define an invaluable resource for measuring oxidative stress in humans.

Original language	English
Publisher	Preprints.org
Number of pages	44
DOIs	https://doi.org/10.20944/preprints202405.0729.v1
Publication status	Published - 12 May 2024

Keywords

oxidative stress
ROS
oxidative damage
redox regulation
antioxidant
method

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10.20944/preprints202405.0729.v1Licence: CC BY

Submitted manuscriptSubmitted manuscript, 2.54 MBLicence: CC BY

Ten “Cheat Codes” for Measuring Oxidative Stress in Humans
Cobley, J. N. (Lead / Corresponding author), Margaritelis, N. V., Chatzinikolaou, P. N., Nikolaidis, M. G. & Davison, G. W., Jul 2024, In: Antioxidants. 13, 7, 38 p., 877.
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abstract = "Formidable and often seemingly insurmountable conceptual, technical, and methodological challenges hamper the measurement of oxidative stress in humans. For instance, fraught and flawed methods, such as the thiobarbituric acid reactive substances assay kits for lipid peroxidation, rate-limit progress. To advance translational redox research, we present ten comprehensive “cheat codes” for measuring oxidative stress in humans. The cheat codes include analytical approaches to assess reactive oxygen species, antioxidants, biomarkers of oxidative damage and redox regulation. They provide essential conceptual, technical, and methodological information inclusive of curated “do” and “don{\textquoteright}t” guidelines. Given the biochemical complexity of oxidative stress, we present a research question-grounded decision tree guide for selecting the most appropriate cheat code (s) to implement in a prospective human experiment. Worked examples demonstrate the benefits of the decision tree-based cheat code selection tool. The ten cheat codes define an invaluable resource for measuring oxidative stress in humans.",

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Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

Abstract

Keywords

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Ten “Cheat Codes” for Measuring Oxidative Stress in Humans

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