Tension between two kinetochores suffices for their bi-orientation on the mitotic spindle

Hilary Dewar, Kozo Tanaka, Kim Nasmyth, Tomoyuki U. Tanaka

    Research output: Contribution to journalArticle

    147 Citations (Scopus)

    Abstract

    The movement of sister chromatids to opposite spindle poles during anaphase depends on the prior capture of sister kinetochores by microtubules with opposing orientations (amphitelic attachment or bi-orientation)1. In addition to proteins necessary for the kinetochore–microtubule attachment, bi-orientation requires the Ipl1 (Aurora B in animal cells) protein kinase2, 3, 4, 5, 6, 7 and tethering of sister chromatids by cohesin8, 9. Syntelic attachments, in which sister kinetochores attach to microtubules with the same orientation, must be either ‘avoided’ or ‘corrected’. Avoidance might be facilitated by the juxtaposition of sister kinetochores such that they face in opposite directions; kinetochore geometry is therefore deemed important. Error correction, by contrast, is thought to stem from the stabilization of kinetochore–spindle pole connections by tension in microtubules, kinetochores, or the surrounding chromatin arising from amphitelic but not syntelic attachment10, 11. The tension model predicts that any type of connection between two kinetochores suffices for efficient bi-orientation. Here we show that the two kinetochores of engineered, unreplicated dicentric chromosomes in Saccharomyces cerevisiae bi-orient efficiently, implying that sister kinetochore geometry is dispensable for bi-orientation. We also show that Ipl1 facilitates bi-orientation by promoting the turnover of kinetochore–spindle pole connections in a tension-dependent manner.
    Original languageEnglish
    Pages (from-to)93-97
    Number of pages5
    JournalNature
    Volume428
    Issue number6978
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Kinetochores
    Spindle Apparatus
    Microtubules
    Chromatids
    Spindle Poles
    Anaphase
    Chromatin
    Saccharomyces cerevisiae
    Proteins
    Chromosomes

    Keywords

    • Kinetochores
    • Microtubules
    • Mitosis

    Cite this

    Dewar, Hilary ; Tanaka, Kozo ; Nasmyth, Kim ; Tanaka, Tomoyuki U. / Tension between two kinetochores suffices for their bi-orientation on the mitotic spindle. In: Nature. 2004 ; Vol. 428, No. 6978. pp. 93-97.
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    Tension between two kinetochores suffices for their bi-orientation on the mitotic spindle. / Dewar, Hilary; Tanaka, Kozo; Nasmyth, Kim; Tanaka, Tomoyuki U.

    In: Nature, Vol. 428, No. 6978, 2004, p. 93-97.

    Research output: Contribution to journalArticle

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