Thapsigargin blocks the induction of long-term potentiation in rat hippocampal slices

Jenni Harvey; Graham L. Collingridge

doi:10.1016/0304-3940(92)90551-H

Thapsigargin blocks the induction of long-term potentiation in rat hippocampal slices

Jenni Harvey (Lead / Corresponding author), Graham L. Collingridge

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143 Citations (Scopus)

Abstract

Experiments were performed to investigate whether intact intracellular Ca²⁺ pools are necessary for long-term potentiation (LTP) in the CA1 region of rat hippocampal slices. Thapsigargin (1 μM), which depletes most intracellular Ca²⁺ pools by blocking ATP-dependent Ca²⁺ uptake into intracellular compartments, blocked the induction but not the expression of LTP. Thapsigargin had no effect on synaptic transmission or on responses mediated by N-methyl-d-aspartate (NMDA) receptor activation. These data suggest that Ca²⁺ release from intracellular stores is required for the induction of LTP.

Original language	English
Pages (from-to)	197-200
Number of pages	4
Journal	Neuroscience Letters
Volume	139
Issue number	2
Early online date	19 Mar 1992
DOIs	https://doi.org/10.1016/0304-3940(92)90551-H
Publication status	Published - 25 May 1992

Keywords

Ca
Ca pool
Hippocampus
LTP
NMDA receptor
Synaptic plasticity

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(92)90551-H

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abstract = "Experiments were performed to investigate whether intact intracellular Ca2+ pools are necessary for long-term potentiation (LTP) in the CA1 region of rat hippocampal slices. Thapsigargin (1 μM), which depletes most intracellular Ca2+ pools by blocking ATP-dependent Ca2+ uptake into intracellular compartments, blocked the induction but not the expression of LTP. Thapsigargin had no effect on synaptic transmission or on responses mediated by N-methyl-d-aspartate (NMDA) receptor activation. These data suggest that Ca2+ release from intracellular stores is required for the induction of LTP.",

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AB - Experiments were performed to investigate whether intact intracellular Ca2+ pools are necessary for long-term potentiation (LTP) in the CA1 region of rat hippocampal slices. Thapsigargin (1 μM), which depletes most intracellular Ca2+ pools by blocking ATP-dependent Ca2+ uptake into intracellular compartments, blocked the induction but not the expression of LTP. Thapsigargin had no effect on synaptic transmission or on responses mediated by N-methyl-d-aspartate (NMDA) receptor activation. These data suggest that Ca2+ release from intracellular stores is required for the induction of LTP.

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KW - Ca pool

KW - Hippocampus

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KW - NMDA receptor

KW - Synaptic plasticity

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Thapsigargin blocks the induction of long-term potentiation in rat hippocampal slices

Abstract

Keywords

ASJC Scopus subject areas

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