The acrosome reaction-inducing effect of human follicular and oviductal fluid

C J De Jonge, C L Barratt, E Radwanska, I D Cooke

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The human sperm acrosome reaction (AR) can be induced in vitro by a variety of naturally occurring and synthetic compounds. The present investigation determined the effect of human natural cycle periovulatory follicular (hFF) and oviductal fluids (hOF) on the human sperm AR in dose-response fashion using the synchronous AR assay. When hFF (30% v/v) or hOF (40% v/v) was added to non-capacitated spermatozoa, no significant (P > 0.05) increase in the % AR was detected in comparison to the non-treatment control. When either of these compounds was added (10%, 20%, and 30% v/v hFF; 20%, 30%, and 40% v/v hOF) to capacitated spermatozoa (3 hour incubation), a significant (P < 0.05) stimulation of the AR was detected. Bovine oviductal fluid (bOF) was tested (20%, 30%, and 40% v/v) to determine if it might have an effect similar to hOF. In contrast to hOF, the highest concentration of bOF (40% v/v) tested failed to stimulate a significant (P > 0.05) increase in the % AR of capacitated spermatozoa in comparison to control. Inhibitors of protein kinases A (KT5720) and C (calphostin C) were tested to determine their effects on the hFF-induced AR. In comparison to hFF treatment alone, the kinase A inhibitor KT5720 (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR when added at the end of the capacitation period and 5 minutes prior to the addition of inducer. Similarly, the kinase C inhibitor calphostin C (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR. The present data demonstrate that periovulatory hFF and hOF stimulate the human sperm AR.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)359-65
Number of pages7
JournalJournal of Andrology
Volume14
Issue number5
DOIs
Publication statusPublished - 1 Sep 1993

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Acrosome Reaction
Follicular Fluid
Spermatozoa
Phosphotransferases
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C

Keywords

  • Acrosome/physiology
  • Animals
  • Carbazoles
  • Cattle
  • Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
  • Fallopian Tubes/physiology
  • Female
  • Follicular Fluid/physiology
  • Humans
  • Indoles/pharmacology
  • Male
  • Naphthalenes
  • Polycyclic Compounds/pharmacology
  • Protein Kinase C/antagonists & inhibitors
  • Pyrroles/pharmacology
  • Sperm-Ovum Interactions/drug effects

Cite this

De Jonge, C J ; Barratt, C L ; Radwanska, E ; Cooke, I D. / The acrosome reaction-inducing effect of human follicular and oviductal fluid. In: Journal of Andrology. 1993 ; Vol. 14, No. 5. pp. 359-65.
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abstract = "The human sperm acrosome reaction (AR) can be induced in vitro by a variety of naturally occurring and synthetic compounds. The present investigation determined the effect of human natural cycle periovulatory follicular (hFF) and oviductal fluids (hOF) on the human sperm AR in dose-response fashion using the synchronous AR assay. When hFF (30{\%} v/v) or hOF (40{\%} v/v) was added to non-capacitated spermatozoa, no significant (P > 0.05) increase in the {\%} AR was detected in comparison to the non-treatment control. When either of these compounds was added (10{\%}, 20{\%}, and 30{\%} v/v hFF; 20{\%}, 30{\%}, and 40{\%} v/v hOF) to capacitated spermatozoa (3 hour incubation), a significant (P < 0.05) stimulation of the AR was detected. Bovine oviductal fluid (bOF) was tested (20{\%}, 30{\%}, and 40{\%} v/v) to determine if it might have an effect similar to hOF. In contrast to hOF, the highest concentration of bOF (40{\%} v/v) tested failed to stimulate a significant (P > 0.05) increase in the {\%} AR of capacitated spermatozoa in comparison to control. Inhibitors of protein kinases A (KT5720) and C (calphostin C) were tested to determine their effects on the hFF-induced AR. In comparison to hFF treatment alone, the kinase A inhibitor KT5720 (50 nM, 100 nM) prevented hFF (20{\%}) stimulation of the AR when added at the end of the capacitation period and 5 minutes prior to the addition of inducer. Similarly, the kinase C inhibitor calphostin C (50 nM, 100 nM) prevented hFF (20{\%}) stimulation of the AR. The present data demonstrate that periovulatory hFF and hOF stimulate the human sperm AR.(ABSTRACT TRUNCATED AT 250 WORDS)",
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The acrosome reaction-inducing effect of human follicular and oviductal fluid. / De Jonge, C J; Barratt, C L; Radwanska, E; Cooke, I D.

In: Journal of Andrology, Vol. 14, No. 5, 01.09.1993, p. 359-65.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The acrosome reaction-inducing effect of human follicular and oviductal fluid

AU - De Jonge, C J

AU - Barratt, C L

AU - Radwanska, E

AU - Cooke, I D

PY - 1993/9/1

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N2 - The human sperm acrosome reaction (AR) can be induced in vitro by a variety of naturally occurring and synthetic compounds. The present investigation determined the effect of human natural cycle periovulatory follicular (hFF) and oviductal fluids (hOF) on the human sperm AR in dose-response fashion using the synchronous AR assay. When hFF (30% v/v) or hOF (40% v/v) was added to non-capacitated spermatozoa, no significant (P > 0.05) increase in the % AR was detected in comparison to the non-treatment control. When either of these compounds was added (10%, 20%, and 30% v/v hFF; 20%, 30%, and 40% v/v hOF) to capacitated spermatozoa (3 hour incubation), a significant (P < 0.05) stimulation of the AR was detected. Bovine oviductal fluid (bOF) was tested (20%, 30%, and 40% v/v) to determine if it might have an effect similar to hOF. In contrast to hOF, the highest concentration of bOF (40% v/v) tested failed to stimulate a significant (P > 0.05) increase in the % AR of capacitated spermatozoa in comparison to control. Inhibitors of protein kinases A (KT5720) and C (calphostin C) were tested to determine their effects on the hFF-induced AR. In comparison to hFF treatment alone, the kinase A inhibitor KT5720 (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR when added at the end of the capacitation period and 5 minutes prior to the addition of inducer. Similarly, the kinase C inhibitor calphostin C (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR. The present data demonstrate that periovulatory hFF and hOF stimulate the human sperm AR.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - The human sperm acrosome reaction (AR) can be induced in vitro by a variety of naturally occurring and synthetic compounds. The present investigation determined the effect of human natural cycle periovulatory follicular (hFF) and oviductal fluids (hOF) on the human sperm AR in dose-response fashion using the synchronous AR assay. When hFF (30% v/v) or hOF (40% v/v) was added to non-capacitated spermatozoa, no significant (P > 0.05) increase in the % AR was detected in comparison to the non-treatment control. When either of these compounds was added (10%, 20%, and 30% v/v hFF; 20%, 30%, and 40% v/v hOF) to capacitated spermatozoa (3 hour incubation), a significant (P < 0.05) stimulation of the AR was detected. Bovine oviductal fluid (bOF) was tested (20%, 30%, and 40% v/v) to determine if it might have an effect similar to hOF. In contrast to hOF, the highest concentration of bOF (40% v/v) tested failed to stimulate a significant (P > 0.05) increase in the % AR of capacitated spermatozoa in comparison to control. Inhibitors of protein kinases A (KT5720) and C (calphostin C) were tested to determine their effects on the hFF-induced AR. In comparison to hFF treatment alone, the kinase A inhibitor KT5720 (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR when added at the end of the capacitation period and 5 minutes prior to the addition of inducer. Similarly, the kinase C inhibitor calphostin C (50 nM, 100 nM) prevented hFF (20%) stimulation of the AR. The present data demonstrate that periovulatory hFF and hOF stimulate the human sperm AR.(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Acrosome/physiology

KW - Animals

KW - Carbazoles

KW - Cattle

KW - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors

KW - Fallopian Tubes/physiology

KW - Female

KW - Follicular Fluid/physiology

KW - Humans

KW - Indoles/pharmacology

KW - Male

KW - Naphthalenes

KW - Polycyclic Compounds/pharmacology

KW - Protein Kinase C/antagonists & inhibitors

KW - Pyrroles/pharmacology

KW - Sperm-Ovum Interactions/drug effects

U2 - 10.1002/j.1939-4640.1993.tb00400.x

DO - 10.1002/j.1939-4640.1993.tb00400.x

M3 - Article

VL - 14

SP - 359

EP - 365

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 5

ER -