The adenomatous polyposis coli protein (APC) exists in two distinct soluble complexes with different functions

TY - JOUR

T1 - The adenomatous polyposis coli protein (APC) exists in two distinct soluble complexes with different functions

AU - Penman, George A.

AU - Leung, Louie

AU - Näthke, Inke S.

PY - 2005/10

Y1 - 2005/10

N2 - Mutations resulting in the truncation of the adenomatous polyposis coli (APQ protein are common to most colonic tumours. The APC protein has emerged as a multifunctional protein that contributes to cytoskeletal organisation and is involved in the regulation of β-catenin. Both, changes in transcription due to increases in β-catenin, as well as defects in directed cell migration and cell division contribute to cancer when APC is mutated. Little is known about how separate functions of APC are coordinated. In this study, we identified two distinct soluble protein pools containing APC. We found that one of these pools represents the fully assembled β-catenin-targeting complex. The second pool contained at least two different forms of APC: APC that was bound to partially assembled β-catenin-targeting complexes and APC that could bind microtubules. Consistent with the previously proposed role for glycogen synthase kinase 3β (GSK3β) in modulating the assembly and activity of the β-catenin-targeting complex, formation of the fully assembled complex was reduced by inhibitors of GSK3β. Similarly, tumour cells with truncated APC only contained the partially assembly β-catenin-targeting complex. We also found that highly elevated levels of β-catenin in tumour cells containing wild-type APC correlated with a decrease in the ability of the endogenous APC protein to bind microtubules. Additionally, APC lacking the direct microtubule binding site was more effective at downregulating β-catenin. Together, our data suggest that the interaction of APC with microtubules and the β-catenin-targeting complex are mutually exclusive, and indicate that the distribution of endogenous APC between different pools is dynamic, which allows cells to distribute it as required.

AB - Mutations resulting in the truncation of the adenomatous polyposis coli (APQ protein are common to most colonic tumours. The APC protein has emerged as a multifunctional protein that contributes to cytoskeletal organisation and is involved in the regulation of β-catenin. Both, changes in transcription due to increases in β-catenin, as well as defects in directed cell migration and cell division contribute to cancer when APC is mutated. Little is known about how separate functions of APC are coordinated. In this study, we identified two distinct soluble protein pools containing APC. We found that one of these pools represents the fully assembled β-catenin-targeting complex. The second pool contained at least two different forms of APC: APC that was bound to partially assembled β-catenin-targeting complexes and APC that could bind microtubules. Consistent with the previously proposed role for glycogen synthase kinase 3β (GSK3β) in modulating the assembly and activity of the β-catenin-targeting complex, formation of the fully assembled complex was reduced by inhibitors of GSK3β. Similarly, tumour cells with truncated APC only contained the partially assembly β-catenin-targeting complex. We also found that highly elevated levels of β-catenin in tumour cells containing wild-type APC correlated with a decrease in the ability of the endogenous APC protein to bind microtubules. Additionally, APC lacking the direct microtubule binding site was more effective at downregulating β-catenin. Together, our data suggest that the interaction of APC with microtubules and the β-catenin-targeting complex are mutually exclusive, and indicate that the distribution of endogenous APC between different pools is dynamic, which allows cells to distribute it as required.

KW - β-Catenin

KW - APC

KW - Microtubules

KW - Protein complexes

UR - https://www.scopus.com/pages/publications/27844450521

U2 - 10.1242/jcs.02589

DO - 10.1242/jcs.02589

M3 - Article

C2 - 16188939

AN - SCOPUS:27844450521

SN - 0021-9533

VL - 118

SP - 4741

EP - 4750

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 20

ER -