TY - CHAP
T1 - The application of LTR retrotransposons as molecular markers in plants
AU - Schulman, Alan H.
AU - Flavell, Alan J.
AU - Paux, Etienne
AU - Ellis, T. H. Noel
PY - 2012
Y1 - 2012
N2 - Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction.
AB - Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction.
UR - http://www.scopus.com/inward/record.url?scp=84860176827&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-603-6_7
DO - 10.1007/978-1-61779-603-6_7
M3 - Chapter (peer-reviewed)
C2 - 22367869
AN - SCOPUS:84860176827
SN - 9781617796029
T3 - Methods in Molecular Biology
SP - 115
EP - 153
BT - Mobile genetic elements
A2 - Walker, John M.
A2 - Bigot, Yves
PB - Humana Press
CY - New York
ER -