The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis

Sarah M. McKim, Grethe-Elisabeth Stenvik, Melinka A. Butenko, Wenche Kristiansen, Sung Ki Cho, Shelley R. Hepworth, Reidunn B. Aalen, George W. Haughn (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    76 Citations (Scopus)

    Abstract

    The Arabidopsis BLADE-ON-PETIOLE 1 (BOP1) and BOP2 genes encode redundant transcription factors that promote morphological
    asymmetry during leaf and floral development. Loss-of-function bop1 bop2 mutants display a range of developmental defects,
    including a loss of floral organ abscission. Abscission occurs along specialised cell files, called abscission zones (AZs) that develop at
    the junction between the leaving organ and main plant body. We have characterized the bop1 bop2 abscission phenotype to
    determine how BOP1 and BOP2 contribute to the known abscission developmental framework. Histological analysis and petal
    breakstrength measurements of bop1 bop2 flowers show no differentiation of floral AZs. Furthermore, vestigial cauline leaf AZs
    are also undifferentiated in bop1 bop2 mutants, suggesting that BOP proteins are essential to establish AZ cells in different tissues.
    In support of this hypothesis, BOP1/BOP2 activity is required for both premature floral organ abscission and the ectopic abscission of
    cauline leaves promoted by the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene under the control of the constitutive CaMV
    35S promoter. Expression of several abscission-related marker genes, including IDA, is relatively unperturbed in bop1 bop2 mutants,
    indicating that these AZ genes respond to positional cues that are independent of BOP1/BOP2 activity. We also show that BOP1 and
    BOP2 promote growth of nectary glands, which normally develop at the receptacle adjacent to developing AZs. Taken together,
    these data suggest that BOP1/BOP2 activity is required for multiple cell differentiation events in the proximal regions of
    inflorescence lateral organs.
    Original languageEnglish
    Pages (from-to)1537-1546
    JournalDevelopment
    Volume135
    Issue number8
    Early online date13 Mar 2008
    DOIs
    Publication statusPublished - 15 Apr 2008

    Fingerprint

    Essential Genes
    Arabidopsis
    Inflorescence
    Genes
    Cues
    Cell Differentiation
    Transcription Factors
    Phenotype
    Growth
    Proteins

    Keywords

    • Abscission
    • BLADE-ON-PETIOLE
    • Flower development
    • INFLORESENCE DEFICIENT IN ABSCISSION

    Cite this

    McKim, S. M., Stenvik, G-E., Butenko, M. A., Kristiansen, W., Cho, S. K., Hepworth, S. R., ... Haughn, G. W. (2008). The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis. Development, 135(8), 1537-1546. https://doi.org/10.1242/dev.012807
    McKim, Sarah M. ; Stenvik, Grethe-Elisabeth ; Butenko, Melinka A. ; Kristiansen, Wenche ; Cho, Sung Ki ; Hepworth, Shelley R. ; Aalen, Reidunn B. ; Haughn, George W. / The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis. In: Development. 2008 ; Vol. 135, No. 8. pp. 1537-1546.
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    abstract = "The Arabidopsis BLADE-ON-PETIOLE 1 (BOP1) and BOP2 genes encode redundant transcription factors that promote morphologicalasymmetry during leaf and floral development. Loss-of-function bop1 bop2 mutants display a range of developmental defects,including a loss of floral organ abscission. Abscission occurs along specialised cell files, called abscission zones (AZs) that develop atthe junction between the leaving organ and main plant body. We have characterized the bop1 bop2 abscission phenotype todetermine how BOP1 and BOP2 contribute to the known abscission developmental framework. Histological analysis and petalbreakstrength measurements of bop1 bop2 flowers show no differentiation of floral AZs. Furthermore, vestigial cauline leaf AZsare also undifferentiated in bop1 bop2 mutants, suggesting that BOP proteins are essential to establish AZ cells in different tissues.In support of this hypothesis, BOP1/BOP2 activity is required for both premature floral organ abscission and the ectopic abscission ofcauline leaves promoted by the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene under the control of the constitutive CaMV35S promoter. Expression of several abscission-related marker genes, including IDA, is relatively unperturbed in bop1 bop2 mutants,indicating that these AZ genes respond to positional cues that are independent of BOP1/BOP2 activity. We also show that BOP1 andBOP2 promote growth of nectary glands, which normally develop at the receptacle adjacent to developing AZs. Taken together,these data suggest that BOP1/BOP2 activity is required for multiple cell differentiation events in the proximal regions ofinflorescence lateral organs.",
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    McKim, SM, Stenvik, G-E, Butenko, MA, Kristiansen, W, Cho, SK, Hepworth, SR, Aalen, RB & Haughn, GW 2008, 'The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis', Development, vol. 135, no. 8, pp. 1537-1546. https://doi.org/10.1242/dev.012807

    The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis. / McKim, Sarah M.; Stenvik, Grethe-Elisabeth; Butenko, Melinka A.; Kristiansen, Wenche; Cho, Sung Ki; Hepworth, Shelley R.; Aalen, Reidunn B.; Haughn, George W. (Lead / Corresponding author).

    In: Development, Vol. 135, No. 8, 15.04.2008, p. 1537-1546.

    Research output: Contribution to journalArticle

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    AU - McKim, Sarah M.

    AU - Stenvik, Grethe-Elisabeth

    AU - Butenko, Melinka A.

    AU - Kristiansen, Wenche

    AU - Cho, Sung Ki

    AU - Hepworth, Shelley R.

    AU - Aalen, Reidunn B.

    AU - Haughn, George W.

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    AB - The Arabidopsis BLADE-ON-PETIOLE 1 (BOP1) and BOP2 genes encode redundant transcription factors that promote morphologicalasymmetry during leaf and floral development. Loss-of-function bop1 bop2 mutants display a range of developmental defects,including a loss of floral organ abscission. Abscission occurs along specialised cell files, called abscission zones (AZs) that develop atthe junction between the leaving organ and main plant body. We have characterized the bop1 bop2 abscission phenotype todetermine how BOP1 and BOP2 contribute to the known abscission developmental framework. Histological analysis and petalbreakstrength measurements of bop1 bop2 flowers show no differentiation of floral AZs. Furthermore, vestigial cauline leaf AZsare also undifferentiated in bop1 bop2 mutants, suggesting that BOP proteins are essential to establish AZ cells in different tissues.In support of this hypothesis, BOP1/BOP2 activity is required for both premature floral organ abscission and the ectopic abscission ofcauline leaves promoted by the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) gene under the control of the constitutive CaMV35S promoter. Expression of several abscission-related marker genes, including IDA, is relatively unperturbed in bop1 bop2 mutants,indicating that these AZ genes respond to positional cues that are independent of BOP1/BOP2 activity. We also show that BOP1 andBOP2 promote growth of nectary glands, which normally develop at the receptacle adjacent to developing AZs. Taken together,these data suggest that BOP1/BOP2 activity is required for multiple cell differentiation events in the proximal regions ofinflorescence lateral organs.

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    McKim SM, Stenvik G-E, Butenko MA, Kristiansen W, Cho SK, Hepworth SR et al. The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis. Development. 2008 Apr 15;135(8):1537-1546. https://doi.org/10.1242/dev.012807