The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli

James W. Donald, Matthew G. Hicks, David J. Richardson, Tracy Palmer

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    Abstract

    We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

    Original languageEnglish
    Pages (from-to)5127-5131
    Number of pages5
    JournalJournal of Bacteriology
    Volume190
    Issue number14
    DOIs
    Publication statusPublished - 2008

    Keywords

    • SHEWANELLA-ONEIDENSIS MR-1
    • II PROTEIN SECRETION
    • DISSIMILATORY FE(III)
    • MN(IV) REDUCTION
    • PATHWAY
    • GENES
    • RESPIRATION
    • TRANSPORT
    • VESICLES
    • COMPLEX

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