The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli

James W. Donald; Matthew G. Hicks; David J. Richardson; Tracy Palmer

doi:10.1128/JB.00395-08

The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli

James W. Donald, Matthew G. Hicks, David J. Richardson, Tracy Palmer

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

Original language	English
Pages (from-to)	5127-5131
Number of pages	5
Journal	Journal of Bacteriology
Volume	190
Issue number	14
DOIs	https://doi.org/10.1128/JB.00395-08
Publication status	Published - 2008

Keywords

SHEWANELLA-ONEIDENSIS MR-1
II PROTEIN SECRETION
DISSIMILATORY FE(III)
MN(IV) REDUCTION
PATHWAY
GENES
RESPIRATION
TRANSPORT
VESICLES
COMPLEX

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title = "The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli",

abstract = "We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.",

keywords = "SHEWANELLA-ONEIDENSIS MR-1, II PROTEIN SECRETION, DISSIMILATORY FE(III), MN(IV) REDUCTION, PATHWAY, GENES, RESPIRATION, TRANSPORT, VESICLES, COMPLEX",

author = "Donald, \{James W.\} and Hicks, \{Matthew G.\} and Richardson, \{David J.\} and Tracy Palmer",

year = "2008",

doi = "10.1128/JB.00395-08",

language = "English",

volume = "190",

pages = "5127--5131",

journal = "Journal of Bacteriology",

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publisher = "American Society for Microbiology",

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T1 - The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli

AU - Donald, James W.

AU - Hicks, Matthew G.

AU - Richardson, David J.

AU - Palmer, Tracy

PY - 2008

Y1 - 2008

N2 - We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

AB - We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

KW - SHEWANELLA-ONEIDENSIS MR-1

KW - II PROTEIN SECRETION

KW - DISSIMILATORY FE(III)

KW - MN(IV) REDUCTION

KW - PATHWAY

KW - GENES

KW - RESPIRATION

KW - TRANSPORT

KW - VESICLES

KW - COMPLEX

U2 - 10.1128/JB.00395-08

DO - 10.1128/JB.00395-08

M3 - Article

C2 - 18487320

SN - 0021-9193

VL - 190

SP - 5127

EP - 5131

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 14

ER -

The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in escherichia coli

Abstract

Keywords

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