The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Ewa Sybilska; Anna Collin; Bahareh Sadat Haddadi; Luis A. J. Mur; Manfred Beckmann; Wenbin Guo; Craig G. Simpson; Agata Daszkowska-Golec

doi:10.1038/s41598-024-69373-9

The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Ewa Sybilska, Anna Collin, Bahareh Sadat Haddadi, Luis A. J. Mur, Manfred Beckmann, Wenbin Guo, Craig G. Simpson, Agata Daszkowska-Golec (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

4 Downloads (Pure)

Abstract

To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

Original language	English
Article number	18278
Number of pages	17
Journal	Scientific Reports
Volume	14
DOIs	https://doi.org/10.1038/s41598-024-69373-9
Publication status	Published - 7 Aug 2024

Keywords

ABA
Alternative splicing
Barley
Cap-binding complex
Embryo
Germination
Transcriptome

ASJC Scopus subject areas

General

Access to Document

10.1038/s41598-024-69373-9Licence: CC BY

Final published versionFinal published version, 3.95 MBLicence: CC BY

Cite this

@article{c23f5d41d88e41cda98cdbfb2da94d06,

title = "The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)",

abstract = "To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.",

keywords = "ABA, Alternative splicing, Barley, Cap-binding complex, Embryo, Germination, Transcriptome",

author = "Ewa Sybilska and Anna Collin and {Sadat Haddadi}, Bahareh and Mur, {Luis A. J.} and Manfred Beckmann and Wenbin Guo and Simpson, {Craig G.} and Agata Daszkowska-Golec",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = aug,

day = "7",

doi = "10.1038/s41598-024-69373-9",

language = "English",

volume = "14",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

AU - Sybilska, Ewa

AU - Collin, Anna

AU - Sadat Haddadi, Bahareh

AU - Mur, Luis A. J.

AU - Beckmann, Manfred

AU - Guo, Wenbin

AU - Simpson, Craig G.

AU - Daszkowska-Golec, Agata

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/8/7

Y1 - 2024/8/7

N2 - To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

AB - To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

KW - ABA

KW - Alternative splicing

KW - Barley

KW - Cap-binding complex

KW - Embryo

KW - Germination

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85200584410&partnerID=8YFLogxK

U2 - 10.1038/s41598-024-69373-9

DO - 10.1038/s41598-024-69373-9

M3 - Article

C2 - 39107424

AN - SCOPUS:85200584410

SN - 2045-2322

VL - 14

JO - Scientific Reports

JF - Scientific Reports

M1 - 18278

ER -

The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this