TY - JOUR
T1 - The chaperone Lhs1 contributes to the virulence of the fish-pathogenic oomycete Aphanomyces invadans
AU - Iberahim, Nurul Aqilah
AU - Sood, Neeraj
AU - Pradhan, Pravata Kumar
AU - van den Boom, Johannes
AU - van West, Pieter
AU - Trusch, Franziska
N1 - The work was carried out with financial support from the BBSRC (BB/M026566/1), UK ESRC and UK Aid under contract BB/N005058/1 (Newton Global Challenge Research Fund (GCRF)) (PvW, FT) and the Department of Biotechnology, Ministry of Science and Technology, Government of India (Grant number BT/IN/Indo-UK/BBSRC-qua/38/MSS/2015–16) (NS, PKP).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Oomycetes are fungal-like eukaryotes and many of them are pathogens that threaten natural ecosystems and cause huge financial losses for the aqua- and agriculture industry. Amongst them, Aphanomyces invadans causes Epizootic Ulcerative Syndrome (EUS) in fish which can be responsible for up to 100% mortality in aquaculture. As other eukaryotic pathogens, in order to establish and promote an infection, A. invadans secretes proteins, which are predicted to overcome host defence mechanisms and interfere with other processes inside the host. We investigated the role of Lhs1 which is part of an ER-resident complex that generally promotes the translocation of proteins from the cytoplasm into the ER for further processing and secretion. Interestingly, proteomic studies reveal that only a subset of virulence factors are affected by the silencing of AiLhs1 in A. invadans indicating various secretion pathways for different proteins. Importantly, changes in the secretome upon silencing of AiLhs1 significantly reduces the virulence of A. invadans in the infection model Galleriamellonella. Furthermore, we show that AiLhs1 is important for the production of zoospores and their cluster formation. This renders proteins required for protein ER translocation as interesting targets for the potential development of alternative disease control strategies in agri- and aquaculture.
AB - Oomycetes are fungal-like eukaryotes and many of them are pathogens that threaten natural ecosystems and cause huge financial losses for the aqua- and agriculture industry. Amongst them, Aphanomyces invadans causes Epizootic Ulcerative Syndrome (EUS) in fish which can be responsible for up to 100% mortality in aquaculture. As other eukaryotic pathogens, in order to establish and promote an infection, A. invadans secretes proteins, which are predicted to overcome host defence mechanisms and interfere with other processes inside the host. We investigated the role of Lhs1 which is part of an ER-resident complex that generally promotes the translocation of proteins from the cytoplasm into the ER for further processing and secretion. Interestingly, proteomic studies reveal that only a subset of virulence factors are affected by the silencing of AiLhs1 in A. invadans indicating various secretion pathways for different proteins. Importantly, changes in the secretome upon silencing of AiLhs1 significantly reduces the virulence of A. invadans in the infection model Galleriamellonella. Furthermore, we show that AiLhs1 is important for the production of zoospores and their cluster formation. This renders proteins required for protein ER translocation as interesting targets for the potential development of alternative disease control strategies in agri- and aquaculture.
KW - Aphanomyces invadans
KW - Aquaculture
KW - Effector
KW - Epizootic ulcerative syndrome (EUS)
KW - Oomycete
KW - Pathogen
KW - Protein secretion
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=85092242695&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2020.09.003
DO - 10.1016/j.funbio.2020.09.003
M3 - Article
C2 - 33213782
SN - 1878-6146
VL - 124
SP - 1024
EP - 1031
JO - Fungal Biology
JF - Fungal Biology
IS - 12
ER -