The chromatin remodelling enzymes SNF2H and SNF2L position nucleosomes adjacent to CTCF and other transcription

Nicola Wiechens (Lead / Corresponding author), Vijender Singh, Triantafyllos Gkikopoulos, Pieta Schofield, Sonia Rocha, Thomas Owen-Hughes

Research output: Contribution to journalArticle

28 Citations (Scopus)
124 Downloads (Pure)

Abstract

Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin remodelling enzyme SNF2H plays a major role organising arrays of nucleosomes adjacent to the binding sites for the architectural transcription factor CTCF sites and acts to promote CTCF binding. At many other factor binding sites SNF2H and the related enzyme SNF2L contribute to nucleosome organisation. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. This suggests that chromatin remodelling ATPase's most closely related to the Drosophila ISWI protein contribute to the function of many human gene regulatory elements.
Original languageEnglish
JournalPLoS Genetics
DOIs
Publication statusPublished - 28 Mar 2016

Fingerprint

nucleosomes
Chromatin Assembly and Disassembly
Nucleosomes
chromatin
binding sites
transcription (genetics)
Binding Sites
enzyme
Transcription Factors
Enzymes
transcription factors
enzymes
Drosophila Proteins
gene
Regulator Genes
regulator genes
adenosinetriphosphatase
Adenosine Triphosphatases
Drosophila
genome

Keywords

  • nucleosome positioning
  • chromatin remodeling
  • CTCF
  • gene regulation
  • chromatin structure

Cite this

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title = "The chromatin remodelling enzymes SNF2H and SNF2L position nucleosomes adjacent to CTCF and other transcription",
abstract = "Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin remodelling enzyme SNF2H plays a major role organising arrays of nucleosomes adjacent to the binding sites for the architectural transcription factor CTCF sites and acts to promote CTCF binding. At many other factor binding sites SNF2H and the related enzyme SNF2L contribute to nucleosome organisation. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. This suggests that chromatin remodelling ATPase's most closely related to the Drosophila ISWI protein contribute to the function of many human gene regulatory elements.",
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year = "2016",
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day = "28",
doi = "10.1371/journal.pgen.1005940",
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The chromatin remodelling enzymes SNF2H and SNF2L position nucleosomes adjacent to CTCF and other transcription. / Wiechens, Nicola (Lead / Corresponding author); Singh, Vijender; Gkikopoulos, Triantafyllos; Schofield, Pieta; Rocha, Sonia; Owen-Hughes, Thomas.

In: PLoS Genetics, 28.03.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The chromatin remodelling enzymes SNF2H and SNF2L position nucleosomes adjacent to CTCF and other transcription

AU - Wiechens, Nicola

AU - Singh, Vijender

AU - Gkikopoulos, Triantafyllos

AU - Schofield, Pieta

AU - Rocha, Sonia

AU - Owen-Hughes, Thomas

PY - 2016/3/28

Y1 - 2016/3/28

N2 - Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin remodelling enzyme SNF2H plays a major role organising arrays of nucleosomes adjacent to the binding sites for the architectural transcription factor CTCF sites and acts to promote CTCF binding. At many other factor binding sites SNF2H and the related enzyme SNF2L contribute to nucleosome organisation. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. This suggests that chromatin remodelling ATPase's most closely related to the Drosophila ISWI protein contribute to the function of many human gene regulatory elements.

AB - Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin remodelling enzyme SNF2H plays a major role organising arrays of nucleosomes adjacent to the binding sites for the architectural transcription factor CTCF sites and acts to promote CTCF binding. At many other factor binding sites SNF2H and the related enzyme SNF2L contribute to nucleosome organisation. The action of SNF2H at CTCF sites is functionally important as depletion of CTCF or SNF2H affects transcription of a common group of genes. This suggests that chromatin remodelling ATPase's most closely related to the Drosophila ISWI protein contribute to the function of many human gene regulatory elements.

KW - nucleosome positioning

KW - chromatin remodeling

KW - CTCF

KW - gene regulation

KW - chromatin structure

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DO - 10.1371/journal.pgen.1005940

M3 - Article

JO - PLoS Genetics

JF - PLoS Genetics

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