The control and importance of hyaluronan synthase expression in palatogenesis

    Research output: Contribution to journalArticle

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    Abstract

    Development of the lip and palate involves a complex series of events that requires the close co-ordination of cell migration, growth, differentiation, and apoptosis. Palatal shelf elevation is considered to be driven by regional accumulation and hydration of glycosoaminoglycans, principally hyaluronan (HA), which provides an intrinsic shelf force, directed by components of the extracellular matrix (ECM). During embryogenesis, the extracellular and pericellular matrix surrounding migrating and proliferating cells is rich in HA. This would suggest that HA may be important in both shelf growth and fusion. TGFß3 plays an important role in palatogenesis and the corresponding homozygous null (TGFß3(-/-)) mouse, exhibits a defect in the fusion of the palatal shelves resulting in clefting of the secondary palate. TGFß3 is expressed at the future medial edge epithelium (MEE) and at the actual edge epithelium during E14.5, suggesting a role for TGFß3 in fusion. This is substantiated by experiments showing that addition of exogenous TGFß3 can "rescue" the cleft palate phenotype in the null mouse. In addition, TGFß1 and TGFß2 can rescue the null mouse palate (in vitro) to near normal fusion. In vivo a TGFß1 knock-in mouse, where the coding region of the TGFß3 gene was replaced with the full-length TGFß1 cDNA, displayed complete fusion at the mid portion of the secondary palate, whereas the anterior and posterior regions failed to fuse appropriately. We present experimental data indicating that the three HA synthase (Has) enzymes are differentially expressed during palatogenesis. Using immunohistochemistry (IHC) and embryo sections from the TGFß3 null mouse at days E13.5 and E14.5, it was established that there was a decrease in expression of Has2 in the mesenchyme and an increase in expression of Has3 in comparison to the wild-type mouse. In vitro data indicate that HA synthesis is affected by addition of exogenous TGFß3. Preliminary data suggests that this increase in HA synthesis, in response to TGFß3, is under the control of the PI3kinase/Akt pathway.
    Original languageEnglish
    Article number10
    Number of pages11
    JournalFrontiers in Physiology
    Volume4
    DOIs
    Publication statusPublished - 2013

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    Hyaluronic Acid
    Palate
    Extracellular Matrix
    Epithelium
    Cleft Palate
    Mesoderm
    Growth
    Lip
    Embryonic Development
    Cell Movement
    hyaluronan synthase
    Embryonic Structures
    Complementary DNA
    Immunohistochemistry
    Apoptosis
    Phenotype
    Enzymes
    Genes
    In Vitro Techniques

    Cite this

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    title = "The control and importance of hyaluronan synthase expression in palatogenesis",
    abstract = "Development of the lip and palate involves a complex series of events that requires the close co-ordination of cell migration, growth, differentiation, and apoptosis. Palatal shelf elevation is considered to be driven by regional accumulation and hydration of glycosoaminoglycans, principally hyaluronan (HA), which provides an intrinsic shelf force, directed by components of the extracellular matrix (ECM). During embryogenesis, the extracellular and pericellular matrix surrounding migrating and proliferating cells is rich in HA. This would suggest that HA may be important in both shelf growth and fusion. TGF{\ss}3 plays an important role in palatogenesis and the corresponding homozygous null (TGF{\ss}3(-/-)) mouse, exhibits a defect in the fusion of the palatal shelves resulting in clefting of the secondary palate. TGF{\ss}3 is expressed at the future medial edge epithelium (MEE) and at the actual edge epithelium during E14.5, suggesting a role for TGF{\ss}3 in fusion. This is substantiated by experiments showing that addition of exogenous TGF{\ss}3 can {"}rescue{"} the cleft palate phenotype in the null mouse. In addition, TGF{\ss}1 and TGF{\ss}2 can rescue the null mouse palate (in vitro) to near normal fusion. In vivo a TGF{\ss}1 knock-in mouse, where the coding region of the TGF{\ss}3 gene was replaced with the full-length TGF{\ss}1 cDNA, displayed complete fusion at the mid portion of the secondary palate, whereas the anterior and posterior regions failed to fuse appropriately. We present experimental data indicating that the three HA synthase (Has) enzymes are differentially expressed during palatogenesis. Using immunohistochemistry (IHC) and embryo sections from the TGF{\ss}3 null mouse at days E13.5 and E14.5, it was established that there was a decrease in expression of Has2 in the mesenchyme and an increase in expression of Has3 in comparison to the wild-type mouse. In vitro data indicate that HA synthesis is affected by addition of exogenous TGF{\ss}3. Preliminary data suggests that this increase in HA synthesis, in response to TGF{\ss}3, is under the control of the PI3kinase/Akt pathway.",
    author = "Galloway, {Jennifer L} and Jones, {Sarah J} and Mossey, {Peter A} and Ellis, {Ian R}",
    year = "2013",
    doi = "10.3389/fphys.2013.00010",
    language = "English",
    volume = "4",
    journal = "Frontiers in Physiology",
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    The control and importance of hyaluronan synthase expression in palatogenesis. / Galloway, Jennifer L; Jones, Sarah J; Mossey, Peter A; Ellis, Ian R.

    In: Frontiers in Physiology, Vol. 4, 10, 2013.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Galloway, Jennifer L

    AU - Jones, Sarah J

    AU - Mossey, Peter A

    AU - Ellis, Ian R

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    AB - Development of the lip and palate involves a complex series of events that requires the close co-ordination of cell migration, growth, differentiation, and apoptosis. Palatal shelf elevation is considered to be driven by regional accumulation and hydration of glycosoaminoglycans, principally hyaluronan (HA), which provides an intrinsic shelf force, directed by components of the extracellular matrix (ECM). During embryogenesis, the extracellular and pericellular matrix surrounding migrating and proliferating cells is rich in HA. This would suggest that HA may be important in both shelf growth and fusion. TGFß3 plays an important role in palatogenesis and the corresponding homozygous null (TGFß3(-/-)) mouse, exhibits a defect in the fusion of the palatal shelves resulting in clefting of the secondary palate. TGFß3 is expressed at the future medial edge epithelium (MEE) and at the actual edge epithelium during E14.5, suggesting a role for TGFß3 in fusion. This is substantiated by experiments showing that addition of exogenous TGFß3 can "rescue" the cleft palate phenotype in the null mouse. In addition, TGFß1 and TGFß2 can rescue the null mouse palate (in vitro) to near normal fusion. In vivo a TGFß1 knock-in mouse, where the coding region of the TGFß3 gene was replaced with the full-length TGFß1 cDNA, displayed complete fusion at the mid portion of the secondary palate, whereas the anterior and posterior regions failed to fuse appropriately. We present experimental data indicating that the three HA synthase (Has) enzymes are differentially expressed during palatogenesis. Using immunohistochemistry (IHC) and embryo sections from the TGFß3 null mouse at days E13.5 and E14.5, it was established that there was a decrease in expression of Has2 in the mesenchyme and an increase in expression of Has3 in comparison to the wild-type mouse. In vitro data indicate that HA synthesis is affected by addition of exogenous TGFß3. Preliminary data suggests that this increase in HA synthesis, in response to TGFß3, is under the control of the PI3kinase/Akt pathway.

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