Projects per year
Abstract
We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes, and mTORC1 selectively repressed and promoted expression of a subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for negative control of the production of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) by mTORC1 in CTLs. mTORC1 repressed PtdIns(3,4,5)P3 production and determined the requirement for mTORC2 in activation of the kinase Akt. Our unbiased proteomic analysis thus provides comprehensive understanding of CTL identity and the control of CTL function by mTORC1.
Original language | English |
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Pages (from-to) | 104-112 |
Number of pages | 9 |
Journal | Nature Immunology |
Volume | 17 |
Issue number | 1 |
Early online date | 9 Nov 2015 |
DOIs | |
Publication status | Published - Jan 2016 |
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Multidimensional Proteomic Analysis of Metabolic Stress & Cellular Phenotypes (Strategic Grant)
1/01/15 → 31/12/19
Project: Research
Profiles
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Cantrell, Doreen
- Cell Signalling and Immunology - Wellcome Trust Principal Research Fellow of Immunology
Person: Academic