Glycosyl-phosphatidylinositol (GPI) membrane anchors are present on a large number of eukaryotic plasma membrane proteins. Some of these anchors can be cleaved with bacterial phosphatidylinositol-specific phospholipases C, and a glycosyl-phosphatidylinositol-specific phospholipase C from Trypanosoma brucei, to reveal an epitope called the cross-reacting determinant. Other glycosyl-phosphatidylinositol anchors are resistant to the action of these enzymes prior to treatment with mild base. A simple method is described for identifying both phospholipase-sensitive and -resistant anchors using anti-cross-reacting determinant antibodies on Western blots. This procedure represents a high-sensitivity general method for the identification of GPI-anchored proteins.
Guther, M. L. S., de Almeida, M. L. C., Rosenberry, T. L., & Ferguson, M. A. J. (1994). The detection of phospholipase-resistant and -sensitive glycosyl-phosphatidylinositol membrane anchors by Western blotting. Analytical Biochemistry, 219(2), 249-255. https://doi.org/10.1006/abio.1994.1264