The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari Eriophyidae)

Rex Brennan, Linzi Jorgensen, Sandra Gordon, Ken Loades, Christine Hackett, Joanne Russell

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    Gall mite (Cecidophyopsis ribis) is the most serious pest of blackcurrant (Ribes nigrum L.), causing the damaging condition known as 'big bud' and also transmitting blackcurrant reversion virus (BRV) within and between plantations. The identification of resistant germplasm is at present a time-consuming and expensive process, dependent on field infestation plots. Resistance based on gene Ce introgressed from gooseberry has been used in UK breeding programmes for blackcurrant. Using a bulked segregant analysis, 90 AFLP primer combinations were screened and a linkage map constructed around the resistance locus controlled by Ce. Sixteen of the primer combinations produced a fragment in the resistant bulked progeny and the gall mite-resistant parent, but not in the susceptible bulked progeny and parent; subsequent testing on individual progeny identified an AFLP fragment closely linked to gall mite resistance. This fragment, designated E41M88-280, was converted to a PCR-based marker based on sequence-specific primers, amplifying only in resistant individuals. Validation of this marker across a range of susceptible and resistant blackcurrant germplasm with different genetic backgrounds confirmed its reliability in the identification of mite-resistant germplasm containing gene Ce. The conversion of an AFLP fragment to a sequence-based PCR marker simplifies its application and therefore increases its utility for selection of mite-resistant germplasm in high-throughput breeding programmes for blackcurrant.
    Original languageEnglish
    Pages (from-to)205-211
    Number of pages7
    JournalTheoretical and Applied Genetics
    Volume118
    Issue number2
    DOIs
    Publication statusPublished - Jan 2009

    Fingerprint

    Cecidophyopsis ribis
    Eriophyidae
    black currants
    Mites
    galls
    mites
    Acari
    germplasm
    Polymerase Chain Reaction
    Ribes
    amplified fragment length polymorphism
    Breeding
    Blackcurrant reversion virus
    Amplified Fragment Length Polymorphism Analysis
    gooseberries
    Ribes nigrum
    breeding
    genetic background
    Genes
    chromosome mapping

    Keywords

    • Amplified Fragment Length Polymorphism Analysis
    • Animals
    • Chromosome Mapping
    • Genetic Linkage
    • Genetic Markers
    • Immunity, Innate
    • Mites
    • Plant Diseases
    • Polymerase Chain Reaction
    • Ribes

    Cite this

    Brennan, Rex ; Jorgensen, Linzi ; Gordon, Sandra ; Loades, Ken ; Hackett, Christine ; Russell, Joanne. / The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari Eriophyidae). In: Theoretical and Applied Genetics. 2009 ; Vol. 118, No. 2. pp. 205-211.
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    abstract = "Gall mite (Cecidophyopsis ribis) is the most serious pest of blackcurrant (Ribes nigrum L.), causing the damaging condition known as 'big bud' and also transmitting blackcurrant reversion virus (BRV) within and between plantations. The identification of resistant germplasm is at present a time-consuming and expensive process, dependent on field infestation plots. Resistance based on gene Ce introgressed from gooseberry has been used in UK breeding programmes for blackcurrant. Using a bulked segregant analysis, 90 AFLP primer combinations were screened and a linkage map constructed around the resistance locus controlled by Ce. Sixteen of the primer combinations produced a fragment in the resistant bulked progeny and the gall mite-resistant parent, but not in the susceptible bulked progeny and parent; subsequent testing on individual progeny identified an AFLP fragment closely linked to gall mite resistance. This fragment, designated E41M88-280, was converted to a PCR-based marker based on sequence-specific primers, amplifying only in resistant individuals. Validation of this marker across a range of susceptible and resistant blackcurrant germplasm with different genetic backgrounds confirmed its reliability in the identification of mite-resistant germplasm containing gene Ce. The conversion of an AFLP fragment to a sequence-based PCR marker simplifies its application and therefore increases its utility for selection of mite-resistant germplasm in high-throughput breeding programmes for blackcurrant.",
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    The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari Eriophyidae). / Brennan, Rex; Jorgensen, Linzi; Gordon, Sandra; Loades, Ken; Hackett, Christine; Russell, Joanne.

    In: Theoretical and Applied Genetics, Vol. 118, No. 2, 01.2009, p. 205-211.

    Research output: Contribution to journalArticle

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    T1 - The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari Eriophyidae)

    AU - Brennan, Rex

    AU - Jorgensen, Linzi

    AU - Gordon, Sandra

    AU - Loades, Ken

    AU - Hackett, Christine

    AU - Russell, Joanne

    PY - 2009/1

    Y1 - 2009/1

    N2 - Gall mite (Cecidophyopsis ribis) is the most serious pest of blackcurrant (Ribes nigrum L.), causing the damaging condition known as 'big bud' and also transmitting blackcurrant reversion virus (BRV) within and between plantations. The identification of resistant germplasm is at present a time-consuming and expensive process, dependent on field infestation plots. Resistance based on gene Ce introgressed from gooseberry has been used in UK breeding programmes for blackcurrant. Using a bulked segregant analysis, 90 AFLP primer combinations were screened and a linkage map constructed around the resistance locus controlled by Ce. Sixteen of the primer combinations produced a fragment in the resistant bulked progeny and the gall mite-resistant parent, but not in the susceptible bulked progeny and parent; subsequent testing on individual progeny identified an AFLP fragment closely linked to gall mite resistance. This fragment, designated E41M88-280, was converted to a PCR-based marker based on sequence-specific primers, amplifying only in resistant individuals. Validation of this marker across a range of susceptible and resistant blackcurrant germplasm with different genetic backgrounds confirmed its reliability in the identification of mite-resistant germplasm containing gene Ce. The conversion of an AFLP fragment to a sequence-based PCR marker simplifies its application and therefore increases its utility for selection of mite-resistant germplasm in high-throughput breeding programmes for blackcurrant.

    AB - Gall mite (Cecidophyopsis ribis) is the most serious pest of blackcurrant (Ribes nigrum L.), causing the damaging condition known as 'big bud' and also transmitting blackcurrant reversion virus (BRV) within and between plantations. The identification of resistant germplasm is at present a time-consuming and expensive process, dependent on field infestation plots. Resistance based on gene Ce introgressed from gooseberry has been used in UK breeding programmes for blackcurrant. Using a bulked segregant analysis, 90 AFLP primer combinations were screened and a linkage map constructed around the resistance locus controlled by Ce. Sixteen of the primer combinations produced a fragment in the resistant bulked progeny and the gall mite-resistant parent, but not in the susceptible bulked progeny and parent; subsequent testing on individual progeny identified an AFLP fragment closely linked to gall mite resistance. This fragment, designated E41M88-280, was converted to a PCR-based marker based on sequence-specific primers, amplifying only in resistant individuals. Validation of this marker across a range of susceptible and resistant blackcurrant germplasm with different genetic backgrounds confirmed its reliability in the identification of mite-resistant germplasm containing gene Ce. The conversion of an AFLP fragment to a sequence-based PCR marker simplifies its application and therefore increases its utility for selection of mite-resistant germplasm in high-throughput breeding programmes for blackcurrant.

    KW - Amplified Fragment Length Polymorphism Analysis

    KW - Animals

    KW - Chromosome Mapping

    KW - Genetic Linkage

    KW - Genetic Markers

    KW - Immunity, Innate

    KW - Mites

    KW - Plant Diseases

    KW - Polymerase Chain Reaction

    KW - Ribes

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    M3 - Article

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    JO - Theoretical and Applied Genetics

    JF - Theoretical and Applied Genetics

    SN - 0040-5752

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    ER -