The directionality of RuvAB-mediated branch migration: in vitro studies with three-armed junctions

Kevin Hiom, Irina R. Tsaneva, Stephen C. West (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    BACKGROUND: The Escherichia coli RuvA and RuvB proteins promote the branch migration of 4-way (Holliday) junctions during genetic recombination. The active complex is a tripartite structure in which RuvA protein is bound to the crossover and is sandwiched between two hexameric rings of RuvB. Branch migration requires ATP hydrolysis and occurs as the DNA passes through each RuvB ring.

    RESULTS: In this work, we have investigated the mechanism by which RuvAB catalyses the branch migration of a three-armed (Y) junction. Using synthetic DNA structures, we observed the formation of DNA products, a partial duplex DNA molecule and a single-stranded oligonucleotide, indicative of a branch migration reaction that occurred with unique polarity. Analysis of the RuvAB-junction complex by DNase footprinting showed that RuvA bound asymmetrically to the junction and targeted a single hexameric RuvB ring to one arm of DNA.

    CONCLUSION: Branch migration of a three-armed junction occurs in a unidirectional manner that is determined by the assembly of a single RuvB ring onto one arm of the DNA. The asymmetry of the complex and observed directionality of branch migration indicate that strand passage occurs as the DNA is pulled into the RuvB ring structure, a reaction likely to be coupled with DNA unwinding.

    Original languageEnglish
    Pages (from-to)443-451
    Number of pages9
    JournalGenes to Cells
    Volume1
    Issue number5
    DOIs
    Publication statusPublished - May 1996

    Fingerprint

    DNA
    Cruciform DNA
    Deoxyribonucleases
    In Vitro Techniques
    Oligonucleotides
    Genetic Recombination
    Proteins
    Hydrolysis
    Adenosine Triphosphate
    Escherichia coli

    Keywords

    • Bacterial proteins
    • Base sequence
    • Binding sites
    • DNA footprinting
    • DNA helicases
    • DNA, Bacterial
    • DNA-binding proteins
    • Deoxyribonuclease I
    • Escherichia coli
    • Escherichia coli proteins
    • Models, Molecular
    • Molecular sequence data
    • Molecular structure
    • Nucleic acid conformation
    • Protein conformation
    • Recombination, Genetic

    Cite this

    Hiom, Kevin ; Tsaneva, Irina R. ; West, Stephen C. / The directionality of RuvAB-mediated branch migration : in vitro studies with three-armed junctions. In: Genes to Cells. 1996 ; Vol. 1, No. 5. pp. 443-451.
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    abstract = "BACKGROUND: The Escherichia coli RuvA and RuvB proteins promote the branch migration of 4-way (Holliday) junctions during genetic recombination. The active complex is a tripartite structure in which RuvA protein is bound to the crossover and is sandwiched between two hexameric rings of RuvB. Branch migration requires ATP hydrolysis and occurs as the DNA passes through each RuvB ring.RESULTS: In this work, we have investigated the mechanism by which RuvAB catalyses the branch migration of a three-armed (Y) junction. Using synthetic DNA structures, we observed the formation of DNA products, a partial duplex DNA molecule and a single-stranded oligonucleotide, indicative of a branch migration reaction that occurred with unique polarity. Analysis of the RuvAB-junction complex by DNase footprinting showed that RuvA bound asymmetrically to the junction and targeted a single hexameric RuvB ring to one arm of DNA.CONCLUSION: Branch migration of a three-armed junction occurs in a unidirectional manner that is determined by the assembly of a single RuvB ring onto one arm of the DNA. The asymmetry of the complex and observed directionality of branch migration indicate that strand passage occurs as the DNA is pulled into the RuvB ring structure, a reaction likely to be coupled with DNA unwinding.",
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    The directionality of RuvAB-mediated branch migration : in vitro studies with three-armed junctions. / Hiom, Kevin; Tsaneva, Irina R.; West, Stephen C. (Lead / Corresponding author).

    In: Genes to Cells, Vol. 1, No. 5, 05.1996, p. 443-451.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The directionality of RuvAB-mediated branch migration

    T2 - in vitro studies with three-armed junctions

    AU - Hiom, Kevin

    AU - Tsaneva, Irina R.

    AU - West, Stephen C.

    PY - 1996/5

    Y1 - 1996/5

    N2 - BACKGROUND: The Escherichia coli RuvA and RuvB proteins promote the branch migration of 4-way (Holliday) junctions during genetic recombination. The active complex is a tripartite structure in which RuvA protein is bound to the crossover and is sandwiched between two hexameric rings of RuvB. Branch migration requires ATP hydrolysis and occurs as the DNA passes through each RuvB ring.RESULTS: In this work, we have investigated the mechanism by which RuvAB catalyses the branch migration of a three-armed (Y) junction. Using synthetic DNA structures, we observed the formation of DNA products, a partial duplex DNA molecule and a single-stranded oligonucleotide, indicative of a branch migration reaction that occurred with unique polarity. Analysis of the RuvAB-junction complex by DNase footprinting showed that RuvA bound asymmetrically to the junction and targeted a single hexameric RuvB ring to one arm of DNA.CONCLUSION: Branch migration of a three-armed junction occurs in a unidirectional manner that is determined by the assembly of a single RuvB ring onto one arm of the DNA. The asymmetry of the complex and observed directionality of branch migration indicate that strand passage occurs as the DNA is pulled into the RuvB ring structure, a reaction likely to be coupled with DNA unwinding.

    AB - BACKGROUND: The Escherichia coli RuvA and RuvB proteins promote the branch migration of 4-way (Holliday) junctions during genetic recombination. The active complex is a tripartite structure in which RuvA protein is bound to the crossover and is sandwiched between two hexameric rings of RuvB. Branch migration requires ATP hydrolysis and occurs as the DNA passes through each RuvB ring.RESULTS: In this work, we have investigated the mechanism by which RuvAB catalyses the branch migration of a three-armed (Y) junction. Using synthetic DNA structures, we observed the formation of DNA products, a partial duplex DNA molecule and a single-stranded oligonucleotide, indicative of a branch migration reaction that occurred with unique polarity. Analysis of the RuvAB-junction complex by DNase footprinting showed that RuvA bound asymmetrically to the junction and targeted a single hexameric RuvB ring to one arm of DNA.CONCLUSION: Branch migration of a three-armed junction occurs in a unidirectional manner that is determined by the assembly of a single RuvB ring onto one arm of the DNA. The asymmetry of the complex and observed directionality of branch migration indicate that strand passage occurs as the DNA is pulled into the RuvB ring structure, a reaction likely to be coupled with DNA unwinding.

    KW - Bacterial proteins

    KW - Base sequence

    KW - Binding sites

    KW - DNA footprinting

    KW - DNA helicases

    KW - DNA, Bacterial

    KW - DNA-binding proteins

    KW - Deoxyribonuclease I

    KW - Escherichia coli

    KW - Escherichia coli proteins

    KW - Models, Molecular

    KW - Molecular sequence data

    KW - Molecular structure

    KW - Nucleic acid conformation

    KW - Protein conformation

    KW - Recombination, Genetic

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    ER -