TY - JOUR
T1 - The dynamics of coiled bodies in the nucleus of adenovirus-infected cells
AU - Rebelo, Luisa
AU - Almeida, Fátima
AU - Ramos, Carlos
AU - Bohmann, Kerstin
AU - Lamond, Angus I.
AU - Carmo-Fonseca, Maria
PY - 1996/7/1
Y1 - 1996/7/1
N2 - The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The results indicate that adenovirus infection induces the disassembly of coiled bodies and that this effect is probably secondary to the block of host protein synthesis induced by the virus. Furthermore, coiled bodies are shown to be very labile structures, with a half-life of ~2 h after treatment of HeLa cells with protein synthesis inhibitors. After blocking of protein synthesis, p80 coilin was detected in numerous microfoci that do not concentrate snRNP. These structures may represent precursor forms of the coiled body, which goes through a rapid cycle of assembly/disassembly in the nucleus and requires ongoing protein synthesis to reassemble.
AB - The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The results indicate that adenovirus infection induces the disassembly of coiled bodies and that this effect is probably secondary to the block of host protein synthesis induced by the virus. Furthermore, coiled bodies are shown to be very labile structures, with a half-life of ~2 h after treatment of HeLa cells with protein synthesis inhibitors. After blocking of protein synthesis, p80 coilin was detected in numerous microfoci that do not concentrate snRNP. These structures may represent precursor forms of the coiled body, which goes through a rapid cycle of assembly/disassembly in the nucleus and requires ongoing protein synthesis to reassemble.
UR - http://www.scopus.com/inward/record.url?scp=0030035435&partnerID=8YFLogxK
U2 - 10.1091/mbc.7.7.1137
DO - 10.1091/mbc.7.7.1137
M3 - Article
C2 - 8862526
AN - SCOPUS:0030035435
SN - 1059-1524
VL - 7
SP - 1137
EP - 1151
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 7
ER -