The dynamics of coiled bodies in the nucleus of adenovirus-infected cells

Luisa Rebelo, Fátima Almeida, Carlos Ramos, Kerstin Bohmann, Angus I. Lamond, Maria Carmo-Fonseca

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The results indicate that adenovirus infection induces the disassembly of coiled bodies and that this effect is probably secondary to the block of host protein synthesis induced by the virus. Furthermore, coiled bodies are shown to be very labile structures, with a half-life of ~2 h after treatment of HeLa cells with protein synthesis inhibitors. After blocking of protein synthesis, p80 coilin was detected in numerous microfoci that do not concentrate snRNP. These structures may represent precursor forms of the coiled body, which goes through a rapid cycle of assembly/disassembly in the nucleus and requires ongoing protein synthesis to reassemble.

Original languageEnglish
Pages (from-to)1137-1151
Number of pages15
JournalMolecular Biology of the Cell
Volume7
Issue number7
DOIs
Publication statusPublished - 1 Jul 1996

    Fingerprint

Cite this