The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Victor Vasilevich Revin, Natalia Vasilevna Gromova, Elvira Sergeevna Revina, Igor Pavlovich Grunyushkin, Alexander Yurievich Tychkov, Anastasia Yurievna Samonova, Anastasia Nikolaevna Kukina, Alexander Alexandrovich Moskovkin, Jean Christophe Bourdon, Nikolai Zhelev (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

Original languageEnglish
Pages (from-to)1236-1250
Number of pages15
JournalBiotechnology and Biotechnological Equipment
Volume32
Issue number5
Early online date8 Jan 2018
DOIs
Publication statusPublished - 2018

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Hyperoxia
Erythrocytes
Oxygen
Erythrocyte Membrane
Ions
Phospholipids
Hemoglobins
Calcium
Lipids
Raman Spectrum Analysis
Silica Gel
Lipid Bilayers
Pathologic Processes
Glass

Keywords

  • Erythrocytes
  • fatty acids
  • haematoporphyrin
  • haemoglobin
  • hyperoxia
  • phospholipids

Cite this

Revin, V. V., Gromova, N. V., Revina, E. S., Grunyushkin, I. P., Tychkov, A. Y., Samonova, A. Y., ... Zhelev, N. (2018). The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. Biotechnology and Biotechnological Equipment, 32(5), 1236-1250. https://doi.org/10.1080/13102818.2017.1414633
Revin, Victor Vasilevich ; Gromova, Natalia Vasilevna ; Revina, Elvira Sergeevna ; Grunyushkin, Igor Pavlovich ; Tychkov, Alexander Yurievich ; Samonova, Anastasia Yurievna ; Kukina, Anastasia Nikolaevna ; Moskovkin, Alexander Alexandrovich ; Bourdon, Jean Christophe ; Zhelev, Nikolai. / The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. In: Biotechnology and Biotechnological Equipment. 2018 ; Vol. 32, No. 5. pp. 1236-1250.
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title = "The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function",
abstract = "The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.",
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author = "Revin, {Victor Vasilevich} and Gromova, {Natalia Vasilevna} and Revina, {Elvira Sergeevna} and Grunyushkin, {Igor Pavlovich} and Tychkov, {Alexander Yurievich} and Samonova, {Anastasia Yurievna} and Kukina, {Anastasia Nikolaevna} and Moskovkin, {Alexander Alexandrovich} and Bourdon, {Jean Christophe} and Nikolai Zhelev",
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Revin, VV, Gromova, NV, Revina, ES, Grunyushkin, IP, Tychkov, AY, Samonova, AY, Kukina, AN, Moskovkin, AA, Bourdon, JC & Zhelev, N 2018, 'The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function', Biotechnology and Biotechnological Equipment, vol. 32, no. 5, pp. 1236-1250. https://doi.org/10.1080/13102818.2017.1414633

The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. / Revin, Victor Vasilevich; Gromova, Natalia Vasilevna; Revina, Elvira Sergeevna; Grunyushkin, Igor Pavlovich; Tychkov, Alexander Yurievich; Samonova, Anastasia Yurievna; Kukina, Anastasia Nikolaevna; Moskovkin, Alexander Alexandrovich; Bourdon, Jean Christophe; Zhelev, Nikolai (Lead / Corresponding author).

In: Biotechnology and Biotechnological Equipment, Vol. 32, No. 5, 2018, p. 1236-1250.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

AU - Revin, Victor Vasilevich

AU - Gromova, Natalia Vasilevna

AU - Revina, Elvira Sergeevna

AU - Grunyushkin, Igor Pavlovich

AU - Tychkov, Alexander Yurievich

AU - Samonova, Anastasia Yurievna

AU - Kukina, Anastasia Nikolaevna

AU - Moskovkin, Alexander Alexandrovich

AU - Bourdon, Jean Christophe

AU - Zhelev, Nikolai

N1 - This study was supported by the Russian Science Foundation [grant number 15-15-10025].

PY - 2018

Y1 - 2018

N2 - The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

AB - The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

KW - Erythrocytes

KW - fatty acids

KW - haematoporphyrin

KW - haemoglobin

KW - hyperoxia

KW - phospholipids

U2 - 10.1080/13102818.2017.1414633

DO - 10.1080/13102818.2017.1414633

M3 - Article

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JO - Biotechnology and Biotechnological Equipment

JF - Biotechnology and Biotechnological Equipment

SN - 1310-2818

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