The effect of insulin-induced hypoglycaemia on factor VIII:C concentrations and thrombin activity in subjects with type 1 (insulin-dependent) diabetes

S.H. Ibbotson, A. Catto, J.A. Davies, P. J. Grant

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    Abstract

    The effect of insulin-induced hypoglycaemia on plasma coagulant activity was studied in 11 subjects with well-controlled, uncomplicated type 1 diabetes. Thrombin generation was determined in plasma by a computer ex-vivo assisted chromogenic method and by the activated partial thromboplastin time (APTT). In addition, factor VIII:C, thrombin-antithrombin III (TAT) complex and fibrinopeptide A (FPA) levels were measured. Hypoglycaemia induced a rise in mean (SD) factor VIII:C concentrations from a baseline level of 1.13 (0.32) IU/ml to a peak 15 min after onset of symptoms and they remained increased at 90 min [1.54 (0.57) and 1.5 (0.54) IU/ml, p <0.001 respectively]. A corresponding reduction in time to generate 50% maximal thrombin activity occurred from a pre-insulin value of 56 (6) s to a minimum reading of 46 (7) s at 15 min (p <0.001) and remained low at 90 min [48 (6) s, p <0.001]. APTT shortened from 43.3 (4.8) s to 40.1 (4.6) s at 30 min (p <0.001) but did not fall below the normal range (37.6-42.7 s) and no significant changes in TAT or FPA levels were noted. Factor VIII:C correlated inversely with time to generate 50% maximal thrombin activity and APTT (r = -0.580, p <0.001; r = -0.673, p <0.001, n = 66, respectively). The results show that the rise in plasma factor VIII:C levels induced by hypoglycaemia is accompanied by accelerated rates of generation of thrombin in contact-activated plasma, though concentrations of FPA and TAT remain unchanged, although TAT complexes are not a sensitive marker of in vivo thrombin generation.
    Original languageEnglish
    Pages (from-to)243-246
    Number of pages4
    JournalThrombosis and Haemostasis
    Volume73
    Issue number2
    Publication statusPublished - Feb 1995

    Keywords

    • Thrombin
    • Sensitivity and Specificity
    • Acute Disease
    • Analysis of Variance
    • Diabetes Mellitus, Type 1
    • Humans
    • Adult
    • Hypoglycemia
    • Male
    • Insulin
    • Female
    • Factor VIII

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