The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro

Harminder Sehmi, Ryan C Olley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

OBJECTIVES: This study investigated the effect of tooth brushing force on changes in dentine tubule patency in an erosion-toothbrush abrasion model.

METHODS: 60 dentine samples prepared with an artificial smear layer and divided randomly into control (no toothbrush), 100 g, 200 g or 400 g toothbrush groups. They were immersed in 3:1 artificial saliva/NaF 1450 ppm and either brushed (p35 soft tooth brush; 120 strokes) or not brushed. Then samples were subjected to agitated acid challenge (0.3% citric acid pH2.6 for 2 min). Finally, samples were re-brushed. Calibrated software calculated patent dentine tubules that cause DH in confocal microscopy images taken of samples at each stage.

RESULTS: At baseline, mean patent tubules in all samples were 188 (SD54) with no significant inter-group differences. Following first brushing, mean patent tubules decreased using 100 g to 150 (SD32) (p<0.01) and increased using 400 g to 215 (SD45) (p=0.02). Following acid challenge, patent tubules increased to 218 (SD40) in all samples (p<0.01) with no significant inter-group differences. Following further brushing, mean patent tubules decreased using 100 g to 175 (SD72) (p<0.01), but increased with 400 g to 232 (SD52).

CONCLUSIONS: At higher brushing forces (400 g), more tubules were exposed. At lower brushing forces (100 g), tubule patency decreased even post-acidic challenge.

CLINICAL SIGNIFICANCE: It is often recommended to our patients with DH to brush using lighter brushing forces but our understanding of this force on dentine tubule patency is unknown. The management of DH requires lighter brushing forces to reduce the numbers of patent dentine tubules.

Original languageEnglish
Pages (from-to)1442-7
Number of pages6
JournalJournal of Dentistry
Volume43
Issue number12
DOIs
Publication statusPublished - Dec 2015

Fingerprint

Dentin Sensitivity
Dentin
Tooth
Smear Layer
Artificial Saliva
Acids
Confocal Microscopy
Citric Acid
Software
Stroke
In Vitro Techniques

Keywords

  • Journal Article

Cite this

Sehmi, Harminder ; Olley, Ryan C. / The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro. In: Journal of Dentistry. 2015 ; Vol. 43, No. 12. pp. 1442-7.
@article{4a712d0fb15a4bb39060d7109a1d7509,
title = "The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro",
abstract = "OBJECTIVES: This study investigated the effect of tooth brushing force on changes in dentine tubule patency in an erosion-toothbrush abrasion model.METHODS: 60 dentine samples prepared with an artificial smear layer and divided randomly into control (no toothbrush), 100 g, 200 g or 400 g toothbrush groups. They were immersed in 3:1 artificial saliva/NaF 1450 ppm and either brushed (p35 soft tooth brush; 120 strokes) or not brushed. Then samples were subjected to agitated acid challenge (0.3{\%} citric acid pH2.6 for 2 min). Finally, samples were re-brushed. Calibrated software calculated patent dentine tubules that cause DH in confocal microscopy images taken of samples at each stage.RESULTS: At baseline, mean patent tubules in all samples were 188 (SD54) with no significant inter-group differences. Following first brushing, mean patent tubules decreased using 100 g to 150 (SD32) (p<0.01) and increased using 400 g to 215 (SD45) (p=0.02). Following acid challenge, patent tubules increased to 218 (SD40) in all samples (p<0.01) with no significant inter-group differences. Following further brushing, mean patent tubules decreased using 100 g to 175 (SD72) (p<0.01), but increased with 400 g to 232 (SD52).CONCLUSIONS: At higher brushing forces (400 g), more tubules were exposed. At lower brushing forces (100 g), tubule patency decreased even post-acidic challenge.CLINICAL SIGNIFICANCE: It is often recommended to our patients with DH to brush using lighter brushing forces but our understanding of this force on dentine tubule patency is unknown. The management of DH requires lighter brushing forces to reduce the numbers of patent dentine tubules.",
keywords = "Journal Article",
author = "Harminder Sehmi and Olley, {Ryan C}",
note = "Copyright {\circledC} 2015 Elsevier Ltd. All rights reserved.",
year = "2015",
month = "12",
doi = "10.1016/j.jdent.2015.10.014",
language = "English",
volume = "43",
pages = "1442--7",
journal = "Journal of Dentistry",
issn = "0300-5712",
publisher = "Elsevier",
number = "12",

}

The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro. / Sehmi, Harminder; Olley, Ryan C.

In: Journal of Dentistry, Vol. 43, No. 12, 12.2015, p. 1442-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of toothbrush abrasion force on dentine hypersensitivity in-vitro

AU - Sehmi, Harminder

AU - Olley, Ryan C

N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/12

Y1 - 2015/12

N2 - OBJECTIVES: This study investigated the effect of tooth brushing force on changes in dentine tubule patency in an erosion-toothbrush abrasion model.METHODS: 60 dentine samples prepared with an artificial smear layer and divided randomly into control (no toothbrush), 100 g, 200 g or 400 g toothbrush groups. They were immersed in 3:1 artificial saliva/NaF 1450 ppm and either brushed (p35 soft tooth brush; 120 strokes) or not brushed. Then samples were subjected to agitated acid challenge (0.3% citric acid pH2.6 for 2 min). Finally, samples were re-brushed. Calibrated software calculated patent dentine tubules that cause DH in confocal microscopy images taken of samples at each stage.RESULTS: At baseline, mean patent tubules in all samples were 188 (SD54) with no significant inter-group differences. Following first brushing, mean patent tubules decreased using 100 g to 150 (SD32) (p<0.01) and increased using 400 g to 215 (SD45) (p=0.02). Following acid challenge, patent tubules increased to 218 (SD40) in all samples (p<0.01) with no significant inter-group differences. Following further brushing, mean patent tubules decreased using 100 g to 175 (SD72) (p<0.01), but increased with 400 g to 232 (SD52).CONCLUSIONS: At higher brushing forces (400 g), more tubules were exposed. At lower brushing forces (100 g), tubule patency decreased even post-acidic challenge.CLINICAL SIGNIFICANCE: It is often recommended to our patients with DH to brush using lighter brushing forces but our understanding of this force on dentine tubule patency is unknown. The management of DH requires lighter brushing forces to reduce the numbers of patent dentine tubules.

AB - OBJECTIVES: This study investigated the effect of tooth brushing force on changes in dentine tubule patency in an erosion-toothbrush abrasion model.METHODS: 60 dentine samples prepared with an artificial smear layer and divided randomly into control (no toothbrush), 100 g, 200 g or 400 g toothbrush groups. They were immersed in 3:1 artificial saliva/NaF 1450 ppm and either brushed (p35 soft tooth brush; 120 strokes) or not brushed. Then samples were subjected to agitated acid challenge (0.3% citric acid pH2.6 for 2 min). Finally, samples were re-brushed. Calibrated software calculated patent dentine tubules that cause DH in confocal microscopy images taken of samples at each stage.RESULTS: At baseline, mean patent tubules in all samples were 188 (SD54) with no significant inter-group differences. Following first brushing, mean patent tubules decreased using 100 g to 150 (SD32) (p<0.01) and increased using 400 g to 215 (SD45) (p=0.02). Following acid challenge, patent tubules increased to 218 (SD40) in all samples (p<0.01) with no significant inter-group differences. Following further brushing, mean patent tubules decreased using 100 g to 175 (SD72) (p<0.01), but increased with 400 g to 232 (SD52).CONCLUSIONS: At higher brushing forces (400 g), more tubules were exposed. At lower brushing forces (100 g), tubule patency decreased even post-acidic challenge.CLINICAL SIGNIFICANCE: It is often recommended to our patients with DH to brush using lighter brushing forces but our understanding of this force on dentine tubule patency is unknown. The management of DH requires lighter brushing forces to reduce the numbers of patent dentine tubules.

KW - Journal Article

U2 - 10.1016/j.jdent.2015.10.014

DO - 10.1016/j.jdent.2015.10.014

M3 - Article

VL - 43

SP - 1442

EP - 1447

JO - Journal of Dentistry

JF - Journal of Dentistry

SN - 0300-5712

IS - 12

ER -