The Effects of NMDA Antagonists on Neuronal Activity in Cat Spinal Cord Evoked by Acute Inflammation in the Knee Joint

Hans‐Georg ‐G Schaible, Blair D. Grubb, Volker Neugebauer, Maria Oppmann

    Research output: Contribution to journalArticle

    93 Citations (Scopus)

    Abstract

    In α‐chloralose‐anaesthetized, spinalized cats we examined the effects of NMDA antagonists on the discharges of 71 spinal neurons which had afferent input from the knee joint. These neurons were rendered hyperexcitable by acute arthritis in the knee induced by kaolin and carrageenan. They were located in the deep dorsal and ventral horn and some of them had ascending axons. The N‐methyl‐d‐aspartate (NMDA) antagonists ketamine and d‐2‐amino‐5‐phosphonovalerate (AP5), were administered ionophoretically, and ketamine was also administered intravenously. In some of the experiments the antagonists were tested against the agonists NMDA and quisqualate. The effects of the NMDA antagonists consisted of a significant reduction in the resting activity of neurons and/or the responses of the same neurons to mechanical stimulation of the inflamed knee. Intravenous ketamine was most effective in suppressing the resting and mechanically evoked activity in 25 of 26 neurons tested. Ionophoretically applied ketamine had a suppressive effect in 11 of 21 neurons, and AP5 decreased activity in 17 of 24 cells. The reduction in the resting and/or the mechanically evoked discharges was achieved with doses of the antagonists which suppressed the responses to NMDA but not those to quisqualate. These results suggest that NMDA receptors are involved in the enhanced responses and basal activity of spinal neurons induced by inflammation in the periphery.

    Original languageEnglish
    Pages (from-to)981-991
    Number of pages11
    JournalEuropean Journal of Neuroscience
    Volume3
    Issue number10
    DOIs
    Publication statusPublished - 1 Oct 1991

    Fingerprint

    Knee Joint
    Spinal Cord
    Cats
    Inflammation
    Neurons
    Ketamine
    Quisqualic Acid
    Knee
    Kaolin
    Carrageenan
    Arthritis
    Axons

    Keywords

    • arthritis
    • hyperexcitability
    • inflammation
    • NMDA receptors
    • nociception

    Cite this

    Schaible, Hans‐Georg ‐G ; Grubb, Blair D. ; Neugebauer, Volker ; Oppmann, Maria. / The Effects of NMDA Antagonists on Neuronal Activity in Cat Spinal Cord Evoked by Acute Inflammation in the Knee Joint. In: European Journal of Neuroscience. 1991 ; Vol. 3, No. 10. pp. 981-991.
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    The Effects of NMDA Antagonists on Neuronal Activity in Cat Spinal Cord Evoked by Acute Inflammation in the Knee Joint. / Schaible, Hans‐Georg ‐G; Grubb, Blair D.; Neugebauer, Volker; Oppmann, Maria.

    In: European Journal of Neuroscience, Vol. 3, No. 10, 01.10.1991, p. 981-991.

    Research output: Contribution to journalArticle

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