The effects of pyridine nucleotides on the activity of a calcium-activated nonselective cation channel in the rat insulinoma cell line, CRI-G1

V. Reale, C. N. Hales, M. L. J. Ashford

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    The activity of a calcium-activated nonselective (Ca-NS+) channel in a rat insulinoma cell line (CRI-G1) is inhibited by pyridine nucleotides in excised patches. The effects of all four pyridine nucleotides tested, beta-NAD+, beta-NADH, beta-NADP+ and beta-NADPH were very similar when tested at 0.1 mM, and at 1 mM the phosphorylated forms, beta-NADP+ and beta-NADPH, appeared to be slightly more potent than beta-NAD+ and beta-NADH. All the pyridine nucleotides tested reduced both the open state probability of the channel and the number of functional channels observed in a single patch. The application of beta-NAD+, but not of the other nucleotides tested, to the cytoplasmic surface of isolated inside-out patches from CRI-G1 cells opened a novel nonselective cation channel (the beta-NAD(+)-NS+ channel). The activity of this new channel is calcium sensitive and may also be inhibited by AMP.
    Original languageEnglish
    Pages (from-to)299-307
    Number of pages9
    JournalJournal of Membrane Biology
    Volume142
    Issue number3
    DOIs
    Publication statusPublished - 1994

    Keywords

    • Animals
    • Calcium
    • Pancreatic Neoplasms
    • Insulinoma
    • Ion Channels
    • Islets of Langerhans
    • NADP
    • Rats
    • Patch-Clamp Techniques
    • NAD
    • Tumor Cells, Cultured
    • Kinetics
    • Membrane Potentials

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