The effects of selenium and copper deficiencies on glutathione S-transferase and glutathione peroxidase in rat liver

J R Arthur, P C Morrice, F Nicol, S E Beddows, R Boyd, J D Hayes, G J Beckett

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    Abstract

    Selenium (Se) deficiency in rats produced significant increases in the activity of hepatic glutathione S-transferase (GST) with 1-chloro-2,4-dinitrobenzene as substrate and in various GST isoenzymes when determined by radioimmunoassay. These changes is GST activity and concentration were associated with Se deficiency that was severe enough to provoke decreases of over 98% in hepatic Se-containing glutathione peroxidase activity (Se-GSHpx). However, decreases in hepatic Se-GSHpx of 60% induced by copper (Cu) deficiency had no effect on GST activity or concentration. Increased GST activity in Se deficiency has previously been postulated to be a compensatory response to loss of Se-GSHpx, since some GSTs have a non-Se-glutathione peroxidase (non-Se-GSHpx) activity. However, the GST isoenzymes determined in this study, GST Yb1Yb1, GST YcYc and GST YaYa, are known to have up to 30-fold differences in non-Se-GSHpx activity, but they were all significantly increased to a similar extent in the Se-deficient rats.

    Original languageEnglish
    Pages (from-to)539-44
    Number of pages6
    JournalBiochemical Journal
    Volume248
    Issue number2
    Publication statusPublished - 1 Dec 1987

    Keywords

    • Animals
    • Copper/deficiency
    • Cytosol/enzymology
    • Glutathione Peroxidase/metabolism
    • Glutathione Transferase/metabolism
    • Isoenzymes/metabolism
    • Liver/enzymology
    • Male
    • Radioimmunoassay
    • Rats
    • Rats, Inbred Strains
    • Selenium/deficiency

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  • Cite this

    Arthur, J. R., Morrice, P. C., Nicol, F., Beddows, S. E., Boyd, R., Hayes, J. D., & Beckett, G. J. (1987). The effects of selenium and copper deficiencies on glutathione S-transferase and glutathione peroxidase in rat liver. Biochemical Journal, 248(2), 539-44.