The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin

Piotr Romanowski, Mark A. Madine, Alison Rowles, J. Julian Blow, Ronald A. Laskey

    Research output: Contribution to journalArticlepeer-review

    169 Citations (Scopus)

    Abstract

    The origin recognition complex (ORC) and the minichromosome maintenance (MCM) protein complex were initially discovered in yeast and shown to be essential for DNA replication. Homologues of ORC and MCM proteins exist in higher eukaryotes, including Xenopus. The Xenopus MCM proteins and the Xenopus homologues of Saccharomyces cerevisiae Orc 1p and Orc2p (XOrc1 and XOrc2) have recently been shown to be essential for DNA replication. Here, we describe the different but interdependent functions of the ORC and MCM complexes in DNA replication in Xenopus egg extracts.

    Original languageEnglish
    Pages (from-to)1416-1425
    Number of pages10
    JournalCurrent Biology
    Volume6
    Issue number11
    Publication statusPublished - 1 Nov 1996

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