The ERC1 scaffold protein implicated in cell motility drives the assembly of a liquid phase

Kristyna Sala, Agnese Corbetta, Claudia Minici, Diletta Tonoli, David H. Murray, Eugenia Cammarota, Lucrezia Ribolla, Martina Ramella, Riccardo Fesce, Davide Mazza, Massimo Degano, Ivan de Curtis

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)
    146 Downloads (Pure)

    Abstract

    Several cellular processes depend on networks of proteins assembled at specific sites near the plasma membrane. Scaffold proteins assemble these networks by recruiting relevant molecules. The scaffold protein ERC1/ELKS and its partners promote cell migration and invasion, and assemble into dynamic networks at the protruding edge of cells. Here by electron microscopy and single molecule analysis we identify ERC1 as an extended flexible dimer. We found that ERC1 scaffolds form cytoplasmic condensates with a behavior that is consistent with liquid phases that are modulated by a predicted disordered region of ERC1. These condensates specifically host partners of a network relevant to cell motility, including liprin-α1, which was unnecessary for the formation of condensates, but influenced their dynamic behavior. Phase separation at specific sites of the cell periphery may represent an elegant mechanism to control the assembly and turnover of dynamic scaffolds needed for the spatial localization and processing of molecules.

    Original languageEnglish
    Article number13530
    Number of pages14
    JournalScientific Reports
    Volume9
    DOIs
    Publication statusPublished - 19 Sept 2019

    ASJC Scopus subject areas

    • General

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