The evolution of structured illumination microscopy in studies of HIV

Kelly Marno, Lara Al'Zoubi, Matthew Pearson, Markus Posch, Áine McKnight, Ann P. Wheeler

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.

    Original languageEnglish
    Pages (from-to)20-27
    Number of pages8
    JournalMethods
    Volume88
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Human immunodeficiency virus
    • Structured illumination
    • Super-resolution imaging

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