The evolution of structured illumination microscopy in studies of HIV

Kelly Marno; Lara Al'Zoubi; Matthew Pearson; Markus Posch; Áine McKnight; Ann P. Wheeler

doi:10.1016/j.ymeth.2015.06.007

The evolution of structured illumination microscopy in studies of HIV

Kelly Marno, Lara Al'Zoubi, Matthew Pearson, Markus Posch, Áine McKnight, Ann P. Wheeler

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.

Original language	English
Pages (from-to)	20-27
Number of pages	8
Journal	Methods
Volume	88
DOIs	https://doi.org/10.1016/j.ymeth.2015.06.007
Publication status	Published - 2015

Keywords

Human immunodeficiency virus
Structured illumination
Super-resolution imaging

ASJC Scopus subject areas

Molecular Biology
General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ymeth.2015.06.007

Cite this

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title = "The evolution of structured illumination microscopy in studies of HIV",

abstract = "The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.",

keywords = "Human immunodeficiency virus, Structured illumination, Super-resolution imaging",

author = "Kelly Marno and Lara Al'Zoubi and Matthew Pearson and Markus Posch and {\'A}ine McKnight and Wheeler, {Ann P.}",

note = "Funding Information: We thank Dr. Daniel Metcalf, Nikon Instruments and Dr. Nicolas Sergeant, Carl Zeiss Microscopy for their excellent help, advice and discussion in the preparation of this manuscript. The Nikon Imaging Centre Kings College London for provision of scientific advice. Professor Jason Swedlow and Dr. Ian Dobbie for advice. This work was funded by: QMUL Molecular Cellular Medicine research theme, EuroBioimaging and a MRC Next generation Optical Microscopy grant (ESRIC). Publisher Copyright: {\textcopyright} 2015. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2015",

doi = "10.1016/j.ymeth.2015.06.007",

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AU - Marno, Kelly

AU - Al'Zoubi, Lara

AU - Pearson, Matthew

AU - Posch, Markus

AU - McKnight, Áine

AU - Wheeler, Ann P.

N1 - Funding Information: We thank Dr. Daniel Metcalf, Nikon Instruments and Dr. Nicolas Sergeant, Carl Zeiss Microscopy for their excellent help, advice and discussion in the preparation of this manuscript. The Nikon Imaging Centre Kings College London for provision of scientific advice. Professor Jason Swedlow and Dr. Ian Dobbie for advice. This work was funded by: QMUL Molecular Cellular Medicine research theme, EuroBioimaging and a MRC Next generation Optical Microscopy grant (ESRIC). Publisher Copyright: © 2015. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2015

Y1 - 2015

N2 - The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.

AB - The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.

KW - Human immunodeficiency virus

KW - Structured illumination

KW - Super-resolution imaging

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JO - Methods

JF - Methods

ER -

The evolution of structured illumination microscopy in studies of HIV

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ASJC Scopus subject areas

UN SDGs

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Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging

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The evolution of structured illumination microscopy in studies of HIV

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Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging

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