Projects per year
The resolution limit of conventional light microscopy has proven to be limiting for many biological structures such as viruses including Human immunodeficiency virus (HIV). Individual HIV virions are impossible to study using confocal microscopy as they are well below the 200 nm resolution limit of conventional light microscopes. Structured illumination microscopy (SIM) allows a twofold enhancement in image resolution compared to standard widefield illumination and so provides an excellent tool for study of HIV. Viral capsids (CAs) vary between 110 and 146 nm so this study challenges the performance of SIM microscopes. SIM microscopy was first developed in 2000, commercialised in 2007 and rapidly developed. Here we present the changes in capabilities of the SIM microscopes for study of HIV localisation as the instrumentation for structured illumination microscopy has evolved over the past 8 years.
|Number of pages||8|
|Publication status||Published - 2015|
- Human immunodeficiency virus
- Structured illumination
- Super-resolution imaging
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
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- 1 Finished
Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M., McGloin, D., McKenna, S., Storey, K., Swedlow, J. & Weijer, K.
1/01/13 → 31/12/17