In the cereals such as barley and wheat, recombination is skewed towards the telomeric regions of the chromosomes. This results in the finding that large portions of the cereal genomes hardly ever recombine. This negatively impacts on breeding schemes that want to create new allele combinations and replace disadvantageous alleles. Studies are under way to improve recombination in the centromeric regions. At the James Hutton Institute we characterize a collection of barley desynaptic mutants to discover novel genes controlling meiosis with the ultimate aim of being able to modify the pattern of recombination. The following study used a forward genetics approach by fine mapping and cytologically characterizing the desynaptic mutant des12. des12 is a spontaneous mutation, which was found in the cultivar Betzes and has two alleles. Nearisogenic lines (BW233 and BW232) were created by backcrossing the two alleles with the common cultivar Bowman (wt). In the following a segregating F2 BW233xMorex population was initially mapped using the 384 BeadXpress genotyping platform; mapping des12 to the long arm of 7H. Phenotypically des12 is severely semi-sterile (Figure 1) and is characterized by an abnormal synaptonemal complex during prophase I leading to improper chromosome segregation due to missing ‘linking’ chiasmata. The interest was to map and identify the underlying gene as well as to characterize its function during meiosis.
|Publication status||Published - 2014|
|Event||The British Meiosis Meeting - Edinburgh, United Kingdom|
Duration: 24 Mar 2014 → 25 Mar 2014
|Conference||The British Meiosis Meeting|
|Period||24/03/14 → 25/03/14|