The genome of the kinetoplastid parasite, Leishmania major

Alasdair C. Ivens; Christopher S. Peacock; Elizabeth A. Worthey; Lee Murphy; Gautam Aggarwal; Matthew Berriman; Ellen Sisk; Marie Adele Rajandream; Ellen Adlem; Rita Aert; Atashi Anupama; Zina Apostolou; Philip Attipoe; Nathalie Bason; Christopher Bauser; Alfred Beck; Stephen M. Beverley; Gabriella Bianchettin; Katja Borzym; Gordana Bothe; Carlo V. Bruschi; Matt Collins; Eithon Cadag; Laura Ciarloni; Christine Clayton; Richard M.R. Coulson; Ann Cronin; Angela K. Cruz; Robert M. Davies; Javier De Gaudenzi; Deborah E. Dobson; Andreas Duesterhoeft; Gholam Fazelina; Nigel Fosker; Alberto Carlos Frasch; Audrey Fraser; Monika Fuchs; Claudia Gabel; Arlette Goble; André Goffeau; David Harris; Christiane Hertz-Fowler; Helmut Hilbert; David Horn; Yiting Huang; Sven Klages; Andrew Knights; Michael Kube; Natasha Larke; Lyudmila Litvin; Angela Lord; Tin Louie; Marco Marra; David Masuy; Keith Matthews; Shulamit Michaeli; Jeremy C. Mottram; Silke Müller-Auer; Heather Munden; Siri Nelson; Halina Norbertczak; Karen Oliver; Susan O'Neil; Martin Pentony; Thomas M. Pohl; Claire Price; Benedicte Purnelle; Michael A. Quail; Ester Rabbinowitsch; Richard Reinhardt; Michael Rieger; Joel Rinta; Johan Robben; Laura Robertson; Jeronimo C. Ruiz; Simon Rutter; David Saunders; Melanie Schäfer; Jacquie Schein; David C. Schwartz; Kathy Seeger; Amber Seyler; Sarah Sharp; Heesun Shin; Dhileep Sivam; Rob Squares; Steve Squares; Valentina Tosato; Christy Vogt; Guido Volckaert; Rolf Wambutt; Tim Warren; Holger Wedler; John Woodward; Shiguo Zhou; Wolfgang Zimmermann; Deborah F. Smith; Jenefer Mary Blackwell; Kenneth D. Stuart; Bart Barrell; Peter J. Myler

doi:10.1126/science.1112680

The genome of the kinetoplastid parasite, Leishmania major

Alasdair C. Ivens (Lead / Corresponding author)
, Christopher S. Peacock
, Elizabeth A. Worthey
, Lee Murphy
, Gautam Aggarwal
, Matthew Berriman
, Ellen Sisk
, Marie Adele Rajandream
, Ellen Adlem
, Rita Aert
, Atashi Anupama
, Zina Apostolou
, Philip Attipoe
, Nathalie Bason
, Christopher Bauser
, Alfred Beck
, Stephen M. Beverley
, Gabriella Bianchettin
, Katja Borzym
, Gordana Bothe

Carlo V. Bruschi, Matt Collins, Eithon Cadag, Laura Ciarloni, Christine Clayton, Richard M.R. Coulson, Ann Cronin, Angela K. Cruz, Robert M. Davies, Javier De Gaudenzi, Deborah E. Dobson, Andreas Duesterhoeft, Gholam Fazelina, Nigel Fosker, Alberto Carlos Frasch, Audrey Fraser, Monika Fuchs, Claudia Gabel, Arlette Goble, André Goffeau, David Harris, Christiane Hertz-Fowler, Helmut Hilbert, David Horn, Yiting Huang, Sven Klages, Andrew Knights, Michael Kube, Natasha Larke, Lyudmila Litvin, Angela Lord, Tin Louie, Marco Marra, David Masuy, Keith Matthews, Shulamit Michaeli, Jeremy C. Mottram, Silke Müller-Auer, Heather Munden, Siri Nelson, Halina Norbertczak, Karen Oliver, Susan O'Neil, Martin Pentony, Thomas M. Pohl, Claire Price, Benedicte Purnelle, Michael A. Quail, Ester Rabbinowitsch, Richard Reinhardt, Michael Rieger, Joel Rinta, Johan Robben, Laura Robertson, Jeronimo C. Ruiz, Simon Rutter, David Saunders, Melanie Schäfer, Jacquie Schein, David C. Schwartz, Kathy Seeger, Amber Seyler, Sarah Sharp, Heesun Shin, Dhileep Sivam, Rob Squares, Steve Squares, Valentina Tosato, Christy Vogt, Guido Volckaert, Rolf Wambutt, Tim Warren, Holger Wedler, John Woodward, Shiguo Zhou, Wolfgang Zimmermann, Deborah F. Smith, Jenefer Mary Blackwell, Kenneth D. Stuart, Bart Barrell, Peter J. Myler (Lead / Corresponding author)

Biological Chemistry and Drug Discovery

Research output: Contribution to journal › Article › peer-review

1238 Citations (Scopus)

Abstract

Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.

Original language	English
Pages (from-to)	436-442
Number of pages	7
Journal	Science
Volume	309
Issue number	5733
DOIs	https://doi.org/10.1126/science.1112680
Publication status	Published - 15 Jul 2005

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

General

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10.1126/science.1112680

Cite this

Ivens, A. C., Peacock, C. S., Worthey, E. A., Murphy, L., Aggarwal, G., Berriman, M., Sisk, E., Rajandream, M. A., Adlem, E., Aert, R., Anupama, A., Apostolou, Z., Attipoe, P., Bason, N., Bauser, C., Beck, A., Beverley, S. M., Bianchettin, G., Borzym, K., ... Myler, P. J. (2005). The genome of the kinetoplastid parasite, Leishmania major. Science, 309(5733), 436-442. https://doi.org/10.1126/science.1112680

@article{87547251f5144c2aa3abd4173b027718,

title = "The genome of the kinetoplastid parasite, Leishmania major",

abstract = "Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36\% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.",

author = "Ivens, \{Alasdair C.\} and Peacock, \{Christopher S.\} and Worthey, \{Elizabeth A.\} and Lee Murphy and Gautam Aggarwal and Matthew Berriman and Ellen Sisk and Rajandream, \{Marie Adele\} and Ellen Adlem and Rita Aert and Atashi Anupama and Zina Apostolou and Philip Attipoe and Nathalie Bason and Christopher Bauser and Alfred Beck and Beverley, \{Stephen M.\} and Gabriella Bianchettin and Katja Borzym and Gordana Bothe and Bruschi, \{Carlo V.\} and Matt Collins and Eithon Cadag and Laura Ciarloni and Christine Clayton and Coulson, \{Richard M.R.\} and Ann Cronin and Cruz, \{Angela K.\} and Davies, \{Robert M.\} and \{De Gaudenzi\}, Javier and Dobson, \{Deborah E.\} and Andreas Duesterhoeft and Gholam Fazelina and Nigel Fosker and Frasch, \{Alberto Carlos\} and Audrey Fraser and Monika Fuchs and Claudia Gabel and Arlette Goble and Andr{\'e} Goffeau and David Harris and Christiane Hertz-Fowler and Helmut Hilbert and David Horn and Yiting Huang and Sven Klages and Andrew Knights and Michael Kube and Natasha Larke and Lyudmila Litvin and Angela Lord and Tin Louie and Marco Marra and David Masuy and Keith Matthews and Shulamit Michaeli and Mottram, \{Jeremy C.\} and Silke M{\"u}ller-Auer and Heather Munden and Siri Nelson and Halina Norbertczak and Karen Oliver and Susan O'Neil and Martin Pentony and Pohl, \{Thomas M.\} and Claire Price and Benedicte Purnelle and Quail, \{Michael A.\} and Ester Rabbinowitsch and Richard Reinhardt and Michael Rieger and Joel Rinta and Johan Robben and Laura Robertson and Ruiz, \{Jeronimo C.\} and Simon Rutter and David Saunders and Melanie Sch{\"a}fer and Jacquie Schein and Schwartz, \{David C.\} and Kathy Seeger and Amber Seyler and Sarah Sharp and Heesun Shin and Dhileep Sivam and Rob Squares and Steve Squares and Valentina Tosato and Christy Vogt and Guido Volckaert and Rolf Wambutt and Tim Warren and Holger Wedler and John Woodward and Shiguo Zhou and Wolfgang Zimmermann and Smith, \{Deborah F.\} and Blackwell, \{Jenefer Mary\} and Stuart, \{Kenneth D.\} and Bart Barrell and Myler, \{Peter J.\}",

year = "2005",

month = jul,

day = "15",

doi = "10.1126/science.1112680",

language = "English",

volume = "309",

pages = "436--442",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5733",

}

Ivens, AC, Peacock, CS, Worthey, EA, Murphy, L, Aggarwal, G, Berriman, M, Sisk, E, Rajandream, MA, Adlem, E, Aert, R, Anupama, A, Apostolou, Z, Attipoe, P, Bason, N, Bauser, C, Beck, A, Beverley, SM, Bianchettin, G, Borzym, K, Bothe, G, Bruschi, CV, Collins, M, Cadag, E, Ciarloni, L, Clayton, C, Coulson, RMR, Cronin, A, Cruz, AK, Davies, RM, De Gaudenzi, J, Dobson, DE, Duesterhoeft, A, Fazelina, G, Fosker, N, Frasch, AC, Fraser, A, Fuchs, M, Gabel, C, Goble, A, Goffeau, A, Harris, D, Hertz-Fowler, C, Hilbert, H, Horn, D, Huang, Y, Klages, S, Knights, A, Kube, M, Larke, N, Litvin, L, Lord, A, Louie, T, Marra, M, Masuy, D, Matthews, K, Michaeli, S, Mottram, JC, Müller-Auer, S, Munden, H, Nelson, S, Norbertczak, H, Oliver, K, O'Neil, S, Pentony, M, Pohl, TM, Price, C, Purnelle, B, Quail, MA, Rabbinowitsch, E, Reinhardt, R, Rieger, M, Rinta, J, Robben, J, Robertson, L, Ruiz, JC, Rutter, S, Saunders, D, Schäfer, M, Schein, J, Schwartz, DC, Seeger, K, Seyler, A, Sharp, S, Shin, H, Sivam, D, Squares, R, Squares, S, Tosato, V, Vogt, C, Volckaert, G, Wambutt, R, Warren, T, Wedler, H, Woodward, J, Zhou, S, Zimmermann, W, Smith, DF, Blackwell, JM, Stuart, KD, Barrell, B & Myler, PJ 2005, 'The genome of the kinetoplastid parasite, Leishmania major', Science, vol. 309, no. 5733, pp. 436-442. https://doi.org/10.1126/science.1112680

TY - JOUR

T1 - The genome of the kinetoplastid parasite, Leishmania major

AU - Ivens, Alasdair C.

AU - Peacock, Christopher S.

AU - Worthey, Elizabeth A.

AU - Murphy, Lee

AU - Aggarwal, Gautam

AU - Berriman, Matthew

AU - Sisk, Ellen

AU - Rajandream, Marie Adele

AU - Adlem, Ellen

AU - Aert, Rita

AU - Anupama, Atashi

AU - Apostolou, Zina

AU - Attipoe, Philip

AU - Bason, Nathalie

AU - Bauser, Christopher

AU - Beck, Alfred

AU - Beverley, Stephen M.

AU - Bianchettin, Gabriella

AU - Borzym, Katja

AU - Bothe, Gordana

AU - Bruschi, Carlo V.

AU - Collins, Matt

AU - Cadag, Eithon

AU - Ciarloni, Laura

AU - Clayton, Christine

AU - Coulson, Richard M.R.

AU - Cronin, Ann

AU - Cruz, Angela K.

AU - Davies, Robert M.

AU - De Gaudenzi, Javier

AU - Dobson, Deborah E.

AU - Duesterhoeft, Andreas

AU - Fazelina, Gholam

AU - Fosker, Nigel

AU - Frasch, Alberto Carlos

AU - Fraser, Audrey

AU - Fuchs, Monika

AU - Gabel, Claudia

AU - Goble, Arlette

AU - Goffeau, André

AU - Harris, David

AU - Hertz-Fowler, Christiane

AU - Hilbert, Helmut

AU - Horn, David

AU - Huang, Yiting

AU - Klages, Sven

AU - Knights, Andrew

AU - Kube, Michael

AU - Larke, Natasha

AU - Litvin, Lyudmila

AU - Lord, Angela

AU - Louie, Tin

AU - Marra, Marco

AU - Masuy, David

AU - Matthews, Keith

AU - Michaeli, Shulamit

AU - Mottram, Jeremy C.

AU - Müller-Auer, Silke

AU - Munden, Heather

AU - Nelson, Siri

AU - Norbertczak, Halina

AU - Oliver, Karen

AU - O'Neil, Susan

AU - Pentony, Martin

AU - Pohl, Thomas M.

AU - Price, Claire

AU - Purnelle, Benedicte

AU - Quail, Michael A.

AU - Rabbinowitsch, Ester

AU - Reinhardt, Richard

AU - Rieger, Michael

AU - Rinta, Joel

AU - Robben, Johan

AU - Robertson, Laura

AU - Ruiz, Jeronimo C.

AU - Rutter, Simon

AU - Saunders, David

AU - Schäfer, Melanie

AU - Schein, Jacquie

AU - Schwartz, David C.

AU - Seeger, Kathy

AU - Seyler, Amber

AU - Sharp, Sarah

AU - Shin, Heesun

AU - Sivam, Dhileep

AU - Squares, Rob

AU - Squares, Steve

AU - Tosato, Valentina

AU - Vogt, Christy

AU - Volckaert, Guido

AU - Wambutt, Rolf

AU - Warren, Tim

AU - Wedler, Holger

AU - Woodward, John

AU - Zhou, Shiguo

AU - Zimmermann, Wolfgang

AU - Smith, Deborah F.

AU - Blackwell, Jenefer Mary

AU - Stuart, Kenneth D.

AU - Barrell, Bart

AU - Myler, Peter J.

PY - 2005/7/15

Y1 - 2005/7/15

N2 - Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.

AB - Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.

UR - https://www.scopus.com/pages/publications/22244437571

U2 - 10.1126/science.1112680

DO - 10.1126/science.1112680

M3 - Article

C2 - 16020728

AN - SCOPUS:22244437571

SN - 0036-8075

VL - 309

SP - 436

EP - 442

JO - Science

JF - Science

IS - 5733

ER -

The genome of the kinetoplastid parasite, Leishmania major

Abstract

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