The GpIA7 effector from the potato cyst nematode Globodera pallida targets potato EBP1 and interferes with the plant cell cycle programme

Mirela C. Coke, Sophie Mantelin, Peter Thorpe, Catherine J. Lilley, Kathryn M. Wright, Daniel S. Shaw, Adams Chande, John T. Jones, Peter E. Urwin (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

17 Downloads (Pure)

Abstract

The potato cyst nematode Globodera pallida acquires all of its nutrients from an elaborate feeding site that it establishes in a host plant root. Normal development of the root cells is re-programmed in a process coordinated by secreted nematode effector proteins. The biological function of the G. pallida GpIA7 effector was investigated in this study. GpIA7 is specifically expressed in the subventral pharyngeal glands of pre-parasitic stage nematodes. Ectopic expression of GpIA7 in potato plants affected plant growth and development, suggesting a potential role for this effector in feeding site establishment. Potato plants overexpressing GpIA7 were shorter, with reduced tuber weight and delayed flowering. We provide evidence that GpIA7 associates with the plant growth regulator StEBP1 (ErbB-3 epidermal growth factor receptor-binding protein 1). GpIA7 modulates the regulatory function of StEBP1, altering the expression level of downstream target genes, including ribonucleotide reductase 2, cyclin D3;1, and retinoblastoma related 1, which are down-regulated in plants overexpressing GpIA7. We provide an insight into the molecular mechanism used by the nematode to manipulate the host cell cycle and demonstrate that this may rely, at least in part, on hindering the function of host EBP1.

Original languageEnglish
Article numbererab353
Pages (from-to)7301-7315
Number of pages15
JournalJournal of Experimental Botany
Volume72
Issue number20
Early online date26 Jul 2021
DOIs
Publication statusPublished - 26 Oct 2021

Keywords

  • Cell cycle
  • EBP1
  • RBR1
  • cyclin D3;1
  • endoreduplication
  • nematode
  • potato

Fingerprint

Dive into the research topics of 'The GpIA7 effector from the potato cyst nematode <i>Globodera pallida</i> targets potato EBP1 and interferes with the plant cell cycle programme'. Together they form a unique fingerprint.

Cite this