The high-affinity sulphonylurea receptor regulates KATP channels in nerve terminals of the rat motor cortex

Kevin Lee, Alistair K. Dixon, I C Rowe, M L Ashford, P J Richardson

    Research output: Contribution to journalArticle

    45 Citations (Scopus)

    Abstract

    The coexpression of sulphonylurea binding sites and ATP-sensitive K+(KATP) channels was examined in the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. These channels were not detected on neuronal cell bodies, but sulphonylurea-sensitive KATP channels and charybdotoxin-sensitive, large-conductance calcium-activated K+ BKCa channels were detected by patch clamping of fused nerve terminals from the motor cortex. Subcellular fractionation revealed that high-affinity sulphonylurea binding sites were enriched in the nerve terminal fraction, whereas glibenclamide increased calcium-independent glutamate efflux from isolated nerve terminals. It is concluded that neuronal sulphonylurea receptors and KATP channels are functionally linked in the motor cortex and that they are both selectively expressed in nerve terminals, where the KATP channel may serve to limit glutamate release under conditions of metabolic stress.
    Original languageEnglish
    Pages (from-to)2562-2571
    Number of pages10
    JournalJournal of Neurochemistry
    Volume66
    Issue number6
    DOIs
    Publication statusPublished - Jun 1996

    Keywords

    • Glyburide
    • Animals
    • Calcium
    • Sulfonylurea Compounds
    • Glutamic Acid
    • Potassium Channels
    • Rats
    • Motor Cortex
    • Rats, Sprague-Dawley
    • Hypoglycemic Agents
    • Patch-Clamp Techniques
    • Receptors, Drug
    • Tritium
    • Neurons
    • Potassium Channels, Inwardly Rectifying
    • ATP-Binding Cassette Transporters
    • Nerve Endings
    • Adenosine Triphosphate

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