The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies

Yoshiko Nakae (Lead / Corresponding author), Peter J. Stoward

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    Abstract

    Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm(3) muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.

    Original languageEnglish
    Pages (from-to)627-634
    Number of pages8
    JournalHistochemistry and Cell Biology
    Volume146
    Issue number5
    Early online date9 Jul 2016
    DOIs
    Publication statusPublished - Nov 2016

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    Keywords

    • Lipofuscin
    • Quantification
    • Image analysis
    • Skeletal muscle
    • Mdx mouse
    • Muscular dystrophy

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