The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies

Yoshiko Nakae; Peter J. Stoward

doi:10.1007/s00418-016-1462-3

The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies

Yoshiko Nakae (Lead / Corresponding author), Peter J. Stoward

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

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Abstract

Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm(3) muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.

Original language	English
Pages (from-to)	627-634
Number of pages	8
Journal	Histochemistry and Cell Biology
Volume	146
Issue number	5
Early online date	9 Jul 2016
DOIs	https://doi.org/10.1007/s00418-016-1462-3
Publication status	Published - Nov 2016

Keywords

Lipofuscin
Quantification
Image analysis
Skeletal muscle
Mdx mouse
Muscular dystrophy

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Cite this

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abstract = "Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm(3) muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.",

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author = "Yoshiko Nakae and Stoward, {Peter J.}",

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T1 - The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies

AU - Nakae, Yoshiko

AU - Stoward, Peter J.

PY - 2016/11

Y1 - 2016/11

N2 - Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm(3) muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.

AB - Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm(3) muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.

KW - Lipofuscin

KW - Quantification

KW - Image analysis

KW - Skeletal muscle

KW - Mdx mouse

KW - Muscular dystrophy

U2 - 10.1007/s00418-016-1462-3

DO - 10.1007/s00418-016-1462-3

M3 - Article

C2 - 27394427

SN - 0948-6143

VL - 146

SP - 627

EP - 634

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

IS - 5

ER -

The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies

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