The high-resolution crystal structure of a parallel-stranded guanine tetraplex

Gerard Laughlan; Alastair I.H. Murchie; David G. Norman; Madeleine H. Moore; Peter C.E. Moody; David M.J. Lilley; Ben Luisi

doi:10.1126/science.8036494

The high-resolution crystal structure of a parallel-stranded guanine tetraplex

Gerard Laughlan, Alastair I.H. Murchie, David G. Norman, Madeleine H. Moore, Peter C.E. Moody, David M.J. Lilley, Ben Luisi

University of Dundee

Research output: Contribution to journal › Article › peer-review

489 Citations (Scopus)

Abstract

Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG₄T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.

Original language	English
Pages (from-to)	520-524
Number of pages	5
Journal	Science
Volume	265
Issue number	5171
DOIs	https://doi.org/10.1126/science.8036494
Publication status	Published - 22 Jul 1994

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title = "The high-resolution crystal structure of a parallel-stranded guanine tetraplex",

abstract = "Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.",

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T1 - The high-resolution crystal structure of a parallel-stranded guanine tetraplex

AU - Laughlan, Gerard

AU - Murchie, Alastair I.H.

AU - Norman, David G.

AU - Moore, Madeleine H.

AU - Moody, Peter C.E.

AU - Lilley, David M.J.

AU - Luisi, Ben

PY - 1994/7/22

Y1 - 1994/7/22

N2 - Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.

AB - Repeat tracts of guanine bases found in DNA and RNA can form tetraplex structures in the presence of a variety of monovalent cations. Evidence suggests that guanine tetraplexes assume important functions within chromosomal telomeres, immunoglobulin switch regions, and the human immunodeficiency virus genome. The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Sharply resolved sodium cations were found between and within planes of hydrogen-bonded guanine quartets, and an ordered groove hydration was observed. Distinct intra- and intermolecular stacking arrangements were adopted by the guanine quartets. Thymine bases were exclusively involved in making extensive lattice contacts.

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The high-resolution crystal structure of a parallel-stranded guanine tetraplex

Abstract

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